β-榄香烯聚合物胶束的制备工艺研究

Preparation of β-elemene-loaded Nano Polymeric Micelles

目的优化β-榄香烯纳米聚合物胶束的制备工艺。方法以二硬脂酰磷脂酰乙醇胺聚-乙二醇(DSPE-PEG2000)为纳米聚合物胶束的载体材料,采用薄膜水化法,以包封率为考察指标,通过正交试验考察β-榄香烯与DSPE-PEG2000的质量比、水化时间、水化体积、PBS缓冲液的p H值对制备工艺的影响。结果在β-ELE与DSPE-PEG2000的质量比为1∶10,水化体积为5 m L,水化时间为30 min,所用PBS缓冲液的p H值为7.4时,所制得的纳米聚合物胶束形态规则,大小较为均匀,平均粒径为56 nm,平均包封率为88.83%。结论该制备工艺稳定可行,重复性好,可用于β-榄香烯纳米聚合物胶束的制备。

OBJECTIVE To optimize the preparation technology of β-elemene-loaded nano polymeric micelles. METHODS With poly（ethylene glycol-2000）-grafted distearoy lphosphosphatidylethanolamine（DSPE-PEG2000） as the carrier materials, thin film hydration method was used to prepare β-elemene-loaded nano polymeric micelles; With entrapment efficiency of β-elemene as index, orthogonal design was adopted to optimize effects of the ratio of β-elemene and DSPE-PEG2000, volumn and time of hydration, p H of PBS. RESULTS In the optimal conditions, the mass ratio of β-elemene and DSPE-PEG2000 of 1∶10, hydration volume of 5 m L, hydration time of 30 min, PBS buffer p H of 7.4, the prepared nanoparticles were round and regular in shape without adhesions, the average of particle size was 56 nm, the average of entrapment efficiency was 88.83%. CONCLUSION The preparation established in this optimization method is stable and feasible, and it could be used for the preparation of β-elemene-loaded nano polymeric micelles.