HnRNP L调控口腔癌细胞中CD55内含子7的可变剪切的研究

Alternative Splicing of CD55 Intron 7 Is Regulated by HnRNP L in Oral Squamous Cell Carcinoma

目的:研究在口腔癌细胞中剪切因子hnRNP L对其新的靶基因CD55的内含子7的可变剪切的调控作用。方法::应用GEO2R在线软件对GEO数据库中的关于hnRNP L沉默后细胞前体mRNA可变剪切改变的外显子阵列数据进行再分析,发现了一系列hnRNP L的新靶基因,然后在人口腔鳞状细胞癌细胞系CAL 27中应用RNA干扰技术降低hnRNP L的表达,通过逆转录PCR检测CD55内含子7的可变剪切。结果:对外显子阵列数据再分析发现CD55是hnRNP L的靶基因。在CAL 27细胞中抑制hnRNP L的表达时,促进了包含内含子7的CD55亚型的表达。结论:CD55内含子7的保留受到hnRNP L的调控。CD55是hnRNP L在口腔癌细胞中的靶基因。

Objective：To study the effect of splicing factor hnRNP L on alternative splicing of CD55 intron 7,a new target of hnRNP L in CAL 27 cells.Methods：An exon array data about hnRNP L down-regulation in cells in GEO database was re-analyzed with GEO2 Ronline service.HnRNP L was knocked down by using siRNA in oral squamous cell carcinoma（OSCC）CAL 27 cells.Alternative splicing of CD55 intron 7was analyzed by RT-PCR.Results：CD55is a new target of hnRNP L.Knockdown of hnRNP L in CAL 27 cells significantly increased the inclusion of CD55 intron 7.Conclusion：The retention of CD55 intron 7was regulated by hnRNP L.CD55 was the target of hnRNP L in OSCC cells.