高效氯氰菊酯急性暴露中斑马鱼相关酶活性和基因表达变化

Variations of Relevant Enzyme Activities and Genes Expressions in Zebrafish under Acute Exposure to Beta Cypermethrin

高效氯氰菊酯广泛地应用于农业生产及日常生活中的害虫防治,但对水生生物却毒性极高。为探究高效氯氰菊酯对斑马鱼的急性毒性,以总超氧化物歧化酶(总SOD)、过氧化氢酶(CAT)和乙酰胆碱酯酶(ACh E)活性以及相关的基因表达量变化为检测指标,研究其对斑马鱼成鱼的影响。急性毒性试验结果显示,高效氯氰菊酯对斑马鱼属剧毒物质,斑马鱼的急性中毒症状为身体蜷曲、抽搐,鳃盖扇动加快,游动能力减弱,间歇性出现无规律急速游动和撞壁行为。酶活测定结果显示,斑马鱼组织中总SOD、CAT和ACh E活性与高效氯氰菊酯呈现低浓度诱导、高浓度抑制的剂量效应关系;相关基因表达量的检测结果显示,高效氯氰菊酯会诱导斑马鱼肝脏、肠和脑中Sod1、Cat以及Ache的m RNA表达上调。研究表明,高效氯氰菊酯的神经毒性和氧化损伤共同造成了斑马鱼的中毒甚至死亡。

Beta cypermethrin is widely used in agricultural production and pest control in daily life. However, it is highly toxic to aquatic organisms. To explore the acute toxic effects of beta cypermethrin to zebrafish, the activity of total superoxide dismutase （total SOD）, catalase （CAT）, acetylcholin esterase （AChE）, and related gene expression were measured after the exposure of beta cypermethrin. The results of acute tocixity test showed that beta cypermethrin is highly toxic to zebrafish, the symptoms of poisoning include curling, tic, faster flapping of operculum, weaker swimming ability, intermittent appearance of irregular fast moving and striking behaviour. The enzymatic activity of total SOD, CAT, and AChE in zebrafish increased in low dose of beta cypermethrin and decreased in high dose. The results of related gene expression showed that beta cypermethrin up-regulated the mRNA expression of Sod1, Cat, Ache in zebrafish liver, gut, and brain. All the results indicate that the combination effect of neurotoxicity and oxidative damage lead to the toxication even death of zebrafish.