淫羊藿苷减轻MPP^+对MES23.5细胞的神经毒性损伤（英文）

Icariin protects against MPP^+-induced neurotoxicity in MES23.5 cells

淫羊藿苷是传统中药淫羊藿的主要活性成分。研究显示淫羊藿苷可能是一个潜在的抗老年疾病的药物。本研究旨在探讨淫羊藿苷对MPP^+诱导的MES23.5细胞损伤的保护作用及其可能机制。细胞活力检测结果显示MPP^+可剂量依赖性地损伤MES23.5细胞,降低细胞存活率,淫羊藿苷可以显著抑制MPP^+所诱导的细胞毒性作用。此外,免疫细胞化学和免疫印迹结果显示淫羊藿苷可对抗MPP^+引起的酪氨酸羟化酶(TH)阳性细胞数的减少和蛋白水平的降低。流式细胞术结果显示,淫羊藿苷可以逆转MPP^+所导致的线粒体膜电位的降低。Real-time RT-PCR和Western blot结果显示,淫羊藿苷可逆转MPP^+所导致的Bax基因和蛋白表达水平的上调以及Bcl-2基因和蛋白表达水平的下调;同时,淫羊藿苷也可以抑制MPP^+所诱导的cleaved caspase-3蛋白的表达水平。以上结果提示,淫羊藿苷可明显对抗MPP^+对MES23.5细胞的神经毒性作用,其作用机制可能与抗凋亡有关。

Icariin is the major bioactive component of Epimedium and has been demonstrated to be a potential drug for age-related diseases. The present study was aimed to investigate the neuroprotective properties of icariin against 1-methyl-4-phenylpyridinium ion （MPP＋）-induced neurotoxicity in MES23.5 cells and the possible mechanisms. MTT, assay showed that treatment with MPP＋ attenuated the cell viability in a dose-dependent manner in MES23.5 cells. Icariin pretreatment resulted in an enhancement of survival. Immunocytochemistry analysis revealed that icariin treatment attenuated MPP＋-induced loss of tyrosine hydroxylase （TH） positive cells. Meanwhile, Western blot confirmed MPP＋ significantly decreased the TH protein expression, and icariin pretreatment could reverse the toxic effect of MPP＋. Moreover, flow cytometry showed that MPP^-induced decrease of the mitochondrial membrane potential could be partly restored by icariin. Furthermore, real-time RT-PCR and Western blot analysis demonstrated that icariin treatment restored the MPP＋-induced up-regulation of Bax and down-regulation of Bcl-2 mRNA and protein expressions. Western blot data also revealed the inhibitory effect of icariin on MPP＋-induced up-regulation of cleaved caspase-3. These findings provide the evidence that icariin has neuroprotective properties against MPP＋-induced neurotoxicity in MES23.5 cells and the mechanism might be related to the anti-apoptotic action of icariin.