沙蚕对翅碱蓬-降油细菌系统降解菲效果的影响

Effect of Perinereis aibuhitensis on the degradation of phenanthrene in Suaeda heteroptera and oil-degradation bacteria system

选取近岸大型优势底栖动物多毛类双齿围沙蚕(Perinereis aibuhitensis)以及盐沼先锋植物翅碱蓬(Suaeda heteroptera),应用微宇宙方法比较沙蚕、翅碱蓬、降油细菌3种生物类型不同修复组合对菲的降解效果,分析不同修复组合对沉积质细菌数量的影响,揭示沙蚕对翅碱蓬-降油菌去除、降解菲的强化效应.结果表明,不同生物组合对土壤菲的降解率存在明显差异,降解率从高到低依次为沙蚕-翅碱蓬-降油菌>翅碱蓬-降油菌>沙蚕.其中,至实验30 d时,沙蚕-翅碱蓬-降油菌和翅碱蓬-降油菌两种生物组合对菲的总降解率分别达到97.02%和93.93%;不同生物组合平均降解率均随着时间的延长出现下降趋势;沙蚕对菲去除途径(翅碱蓬-降油菌交互作用、非生物因素)有显著的强化作用,最高强化贡献率分别达到14.32%、20.08%.在0-5 d沙蚕-翅碱蓬-降油菌组合中土壤降油菌数量显著高于翅碱蓬-降油菌组合;随着时间延长,两种生物组合降油菌数量均出现下降趋势,至实验20-30 d时均降至对照组水平.综上可知,沙蚕-翅碱蓬-降油菌联合修复模式较其它模式修复效果更显著,沙蚕对各去除因子降解以菲为代表的多环芳烃(PAHs)有明显的强化作用,并对降油菌生长有促进效果.

bstract This study aimed to investigate the enhancement of marine polychaetes in phenanthrene degradation.We chose Perinereis aibuhitensis,Suaeda heteroptera and oil-degradation bacteria as experimental subjects and established three different combined bioremediation models to examine with wetland microcosm method the depredating rate of phenanthrene as well as bacteria number in sediment among different bioremediation models.The results showed significant different degradation rate of phenanthrene in three bioremediation modes as nereid–suaeda–bacteria suaeda–bacteria nereid.At 30 d the degradation rate in nereid–suaeda–bacteria and suaeda–bacteria model reached 97.02% and 93.93%,respectively.The average degradation rate in each model decreased with time.P.aibuhitensis significantly enhanced the remediation of phenanthrene in terms of suaeda–bacteria interaction and abiotic loss,contributing to 14.32% and 20.08% of their respective increase.At 0–5 d the total number of oil-degradation bacteria in nereid–suaeda–bacteria model was much more than that in suaeda–bacteria model,but the bacteria number in these two models fell sharply to the level of control at 20–30 d.In conclusion,the remediation effect of nereid–suaeda–bacteria model was more prominent than others,and the existence of marine polychaetes could improve hydrocarbon degradation ability of bacteria in sediment.