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4．1R蛋白在老龄心力衰竭小鼠心肌组织中的表达

Expression of 4.1R protein mRNA in myocardial tissues of elderly mice with heart failure
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摘要 目的探讨4．1R蛋白在老龄心力衰竭小鼠心肌组织中的表达水平。方法将40只老龄雄性健康小鼠均分为心力衰竭组（n=20）和对照组（n=20），常规喂养8周后应用超声评估心功能，处死小鼠后迅速取出心脏，计算左室质量指数，应用逆转录．聚合酶链反应（RT-PCR）检测两组老龄小鼠心肌细胞4．1R蛋白mRNA量，比较两者之间的差别。结果与对照组比较，心力衰竭组老龄小鼠心率加快，左室收缩末期内径（LVESD）、左室舒张末期内径（LVEDD）增大，左室射血分数（LVEF）降低，左室质量指数增加，心肌组织4．1R蛋白mRNA表达量明显增加，差异均有统计学意义（P〈0．05）。结论4．1R蛋白mRNA在老龄心力衰竭小鼠心肌组织上表达量明显增加，提示4．1R蛋白可能与心力衰竭的发生发展有关。 Objective To detect the expression of 4. 1R protein in myocardial tissue of elderly mice with heart failure （HF）. Methods A total of 40 healthy elderly male mice were divided into heart failure group （n = 20 ） and control group （n = 20）. After conventional feeding for eight weeks, using ul- trasound to assess their cardiac function, and their hearts were removed to assess the left ventricular mass index （LVMI） after execution. RT-PCR was used to detect the expression of 4. 1R protein in elderly mice myocardial tissue , and compare the differences between the two group. Results Compared with the control group, the heart rate, LVESD, LVEDD, left ventricular mass index in heart failure group markedly increased, while the LVEF decreased, and the expression of 4. 1R protein mRNA significantly increased in myocardial tissue of the heart failure group, and the differences were significant （P 〈 0. 05 ）. Conclusions The significantly increased expression of 4. 1R protein mRNA in myocardial tissue of eld- erly mice with HF suggests that 4. 1R protein may be related to the occurrence and development of heart failure.
作者 豆倩云 杨国杰 魏子寒 秦鹏 李栋博 唐俊燕
机构地区 郑州大学第一附属医院老年心血管科河南省高等学校临床医学重点学科开放实验室
出处 《中国实用医刊》 2016年第20期5-7,F0004,共4页 Chinese Journal of Practical Medicine
关键词 心力衰竭 4.1R蛋白 老龄小鼠 Heart failure 4. 1R protein Elderly mouse
分类号 R541.6 [医药卫生—心血管疾病]
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参考文献13

  • 1Szab6 T, Scherbakov N, Sandek A, et al. Plasma adiponectin in heart failure with and without cachexia: catabolic signal linking catab- olism, symptomatic status, and prognosis [ J]. Nutr Metab Cardiovsc Dis, 2014, 24( 1 ) :50-56.
  • 2Gallagher PG. Hereditary elliptocytosis: speetrln and protein 4.1R[ J ]. Semin Hemato1,2004,41 (2) : 142-164.
  • 3Kuns R, Kissil JL, Newsham IF, et al. Protein 4.1 B ex-pression is induced in mammary epithelial cells during pregnancy and regulates their proliferation[J]. Oncogene, 2005,24(43) :6502-6515.
  • 4Takabashi Y, Iwai M, Kawai T, et al. Aberrant expression of tumor suppressors CADM1 and 4.1 B in invasive lesions of primary breast cancer [ J ]. Breast Cancer, 2012,19 (3) :242-252.
  • 5Bennett PM, Baines A J, Lecomte MC, et al. Not just a plasma mem- brane protein: in cardiac muscle cells alpha- I1 spectrin also shows a close association with myofibrils [ J ]. J Muscle Res Cell Motil, 2004, 25(2) : 119-126.
  • 6Parra M, Gascard P, Walensky LD, et al. Cloning and characteriza- tion of 4.1G (EPB41L2), a new member of the skeletal protein 4.1 (EPB41) gene family[J]. Genomics, 1998,49(2):298-306.
  • 7Diakowski W, Grzybek M, Sikorski AF. Protein 4.1, a component of the erythrocyte membrane skeleton and its related homologue proteins forming the protein 4. 1/FERM superfamily [ J ]. Folia Histochem Cytobiol, 2006, 44(4) :231-248.
  • 8Sun CX, Robb VA, Gutmann DH. Protein 4.1 tumor suppressors : get- ting a FERM grip on growth regulation [ J ]. J Cell Sci, 2002, 115 ( Pt 21 ) :3991-4000.
  • 9MeGowan E, Pickford F, Kim J, et al. A1342 is essential for Paren- chymal and vascular amyloid deposition in mice[J]. Neuron, 2005, 47(2) :191-199.
  • 10Shi ZT, Afzal V, Coller B, et al. Protein 4.1R-deficient mice are via- ble but have erythroid membrane skeleton ab-normalities [ J ]. J Clin Invest, 1999, 103(3) : 331-340.

二级参考文献13

  • 1Haldeman GA, Croft JB, Giles WH, et al. Hospitalization of patients with heart failure:National Hospital Discharge Sur- vey,1985 to 1995[J]. Am Heart J,1999,137(2) :352.
  • 2Redfield MM ,Jacobsen SJ, Burnett JC, et al. Burden of sys- tolic and diastolic ventricular dysfunction in the communi- ty:appreciating the scope of the heart failure epidemic [ J ]. JAMA ,2003,289 (2) : 194.
  • 3Loehr LR, Rosamond WD, Chang PP,et al. Heart failure in- cidence and survival (from the Atherosclerosis Risk in Communities study) [ J]. Am J Cardiol, 2008, 101 ( 7 ) : 1016.
  • 4De Carcer G, Lallena M J, Correas I. Protein 4. 1 is a component of the nuclear matrix of mammalian cells [ J ]. Biochem J, 1995, 312(Pt 3) :871.
  • 5Kuns R, Kissil JL, Newsham IF, et al. Protein 4.1B ex- pression is induced in mammary epithelial cells during pregnancy and regulates their proliferation[ J]. Oncogene, 2005, 24(43): 6502.
  • 6Takahashi Y, Iwai M, Kawai T, et al. Aberrant expression of tumor suppressors CADM1 and 4.1 B in invasive lesions of primary breast cancer [ J ]. Breast Cancer, 2012, 19 (3) : 242.
  • 7Bennett PM, Baines AJ, Lecomte MC, et al. Not just a plasma membrane protein: in cardiac muscle cells alpha- H spectrin also shows a close association with myofibrils [J]. J Muscle Res Cell Motil, 2004, 25(2) : 119.
  • 8Rezkalla S, Kloner RA, Khatib G, et al. Effect of metoprolol in acute coxsackievirus B3 murine myocarditis [ J ]. J Am Coll Cardiol,1988,12(2) :412.
  • 9Chowdhury D, Tangutur AD, Khatua TN, eta]. A proteomic view of isoprotereno] induced cardiac hypertrophy: prohib- itin identified as a potential biomarker in rats[ J ]. J Trans-lational Med,2013, 11 : 130.
  • 10Parra M, Gascard P,Walensky LD, et al. Cloning and char- acterization of 4. IG ( EPB41L2), a new member of the skeletal protein 4. I ( EPB41 ) gene family [ J ]. Genomics, 1998,49 (2) :298.
中国实用医刊
2016年 第20期

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