摘要
目的探讨A型钾通道5种亚型即Kv1.4、Kv3.4、Kv4.1、Kv4.2和Kv4.3在正常大鼠臂旁外侧核(lateral parabrachial nucleus,LPB)的表达。方法分别采用RT-PCR和实时荧光定量PCR技术检测分布于成年雄性SD大鼠LPBA型钾通道亚型。结果用RT-PCR技术在所有6只大鼠LPB都检测到了Kv3.4、Kv4.2和Kv4.3 mRNA,但在1只大鼠LPB未检测到Kv1.4 mRNA,2只大鼠LPB未检测到Kv4.1 mRNA;实时荧光定量PCR实验显示,在LPB,Kv1.4、Kv3.4、Kv4.2和Kv4.3 mRNA表达显著高于Kv4.1 mRNA,而Kv3.4、Kv4.2、Kv4.3和Kv1.4 mRNA的表达丰度差异无统计学意义(P>0.05),但在1只大鼠LPB未检测到Kv1.4 mRNA。结论在正常大鼠LPB中有A型钾通道mRNA表达,其主要亚型为Kv3.4、Kv4.2和Kv4.3。
Objective To investigate the mRNA expressions of A - type potassium channel subtypes in rat lateral parabrachial nucleus （LPB）. Methods The mRNA levels of Kvl. 4, Kv3.4, Kv5.1, Kv4.2 and Kv4.3 were respectively detected with RT - PCR and fluorescent quantitative real time PCR. Results There were mRNA expressions of Kv3.4, Kv4.2 and Kv4.3 in the LPB of all the tested rats by RT - PCR, while Kvl. 4 and Kv4.1 mRNA was not detected in the LPB of 1/6 and 2/6 rats, respectively. With fluorescent quantitative real time PCR, the mRNAs of Kvl. 4, Kv3.4, Kv4.2 and Kv4.3 were significantly abundant than that of Kv5.1, while the mR- NAs of Kvl. 4, Kv3.4, Kv4.2 and Kv4.3 did not show difference, although Kvl. 4 mRNA was not detected in the LPB in one of the 6 rats. Conclusion The mRNA expressions of A - type potassium channel are detected in rat LPB, where the major subtypes of A - type potassium channels are Kv3.4,Kv4.2 and Kv4.3.
出处
《医学研究杂志》
2016年第10期57-60,共4页
Journal of Medical Research
基金
国家级大学生创新创业训练项目(201413705023)
成都医学院大学生创新实验计划项目(CXXS201406)
发育与再生四川省重点实验室研究基金资助项目(SYS14-004)
关键词
臂旁外侧核
A型钾通道
亚型
Lateral parabrachial neuleus
A -type potassium channels
Subtype
