脑血疏对脑出血大鼠脉络丛上皮细胞中食管癌相关基因4表达的影响

Effect of Naoxueshu on the expression of esopageal cancer related gene 4 in choroid plexus epithelial cells in intracerebral hemorrhage rats

目的探讨脑血疏对脑出血大鼠脉络丛上皮细胞（CPECs）中食管癌相关基因4（ECRG4）表达的影响。方法将无特定病原体级SD大鼠分为脑出血组、实验组和对照组，各10只。脑出血组及实验组均用自体血注入法制备脑出血模型，对照组用等量0．9％氯化钠注射液注入尾状核，不制备脑出血模型。实验组及对照组给予等量脑血疏灌胃治疗，脑出血组给予等量0．9％氯化钠注射液灌胃。分离纯化培养各组CPECs，并应用实时定量聚合酶链反应法和蛋白质印迹法分别检测ECRG4在各组CPECs中的表达情况。结果实验组ECRG4mRNA和蛋白表达均明显高于脑出血组和对照组[（15．503±1．460）比（1．032±0．320）、（1．548±1．390），（5．268±0．750）比（1．000±0．000）、（1．348±0．120）]，脑出血组ECRG4蛋白表达明显低于对照组，差异均有统计学意义（均P〈0．05）。结论ECRG4可能参与了CPECs对脑损伤的反应，并影响了CPECs的生物学特性，早期使用脑血疏可调控ECRG4在CPECs中的表达，为CPECs在神经保护中的作用研究提供了理论依据。

Objective To analyze the effect of Naoxueshu on the expression of esopageal cancer related gene 4 （ ECRG4 ） in choroid plexus epithelial cells （ CPECs ） in intracerebral hemorrhage （ ICH ） rats. Methods Ten rats were included in ICH group, observation group and control group respectively. The ICH group and observation group were ICH models; the control group was injected 0. 9% sodium chloride in caudate nucleus. Observation group and control group were treated with intragastric administration with Naoxueshu; ICH group was treated with intragastric administration with equivalent 0. 9% sodium chloride. The expression of ECRG4 in CPECs was measured by real-time polymerase chain reaction（RT-PCR） and Western blot method. Results Expressions of ECRG4 mRNA and protein in observation group were significantly higher than those in ICH group and control group[ （15. 503 ±1. 460） vs （1.032±0.320）,（1.548 ±1.390）;（5.268 ±0.750） vs （1.000±0.000）, （1. 348 ± 0. 120 ） ] ; the expression of ECRG4 protein in ICH group was significantly lower than that in control group （ all P 〈 0. 05 ）. Conclusions ECRG4 may be related to the reaction of CPECs in cerebral injury and influ- ence biologic characteristics of CPECs. Naoxueshu can regulate the expression of ECRG4 in CPECs, providing an evidence for the neuroprotective effect of CPECs.