摘要
目的:观察vaspin对糖尿病肾病(DN)大鼠肾组织核因子-κB(NF-κB)和转化生长因子-β_1(TGF-β_1)表达的影响。方法:将40只雄性SD大鼠随机分为对照组(10只)和造模组(30只),再将30只造模成功的DN大鼠随机分为3组:DN组、vaspin组、氯沙坦组,每组10只。干预12周后检测大鼠血肌酐(Scr)、尿素氮(BUN)和24 h尿蛋白定量。应用RT-PCR和Western blot分别检测大鼠肾组织NF-κB和TGF-β_1的基因和蛋白表达。结果:DN组大鼠的Scr、BUN和24 h尿蛋白定量均高于对照组(P<0.05),肾组织中NF-κB和TGF-β_1表达水平明显高于对照组(P<0.05);vaspin组大鼠的Scr、BUN和24 h尿蛋白定量均低于DN组(P<0.05),肾组织中NF-κB和TGF-β_1的表达水平明显低于DN组(P<0.05)。结论:Vaspin可能通过下调DN大鼠肾组织NF-κB和TGF-β_1的表达,延缓DN的进展。
Objective ：To investigate the effects of vaspin on expression of NF - κB and TGF - β1 in renal tissue of diabetic nephropathy rats. Methods：40 male SD rats were randomly divided into control group, diabetic nephropathy group, losartan group, vaspin group, 10 in each group. The levels of serum creatinine, urea nitrogen and 24 - hour urine protein excretion were determined by biochemistry. The expression of NF - κB and TGF - β1 in renal tissue were determined by Real - Time PCR and Western blotting. Results：Compared with control group, the expression of NF - κB and TGF - β1 and the levels of serum creatinine, urea nitrogen and 24 - hour urine protein excretion were significantly elevated in diabetic nephropathy group （ P 〈 0.05 ）. Vaspin could significantly de- crease the expression of NF - κB and TGF - β1 and the levels of serum creatinine, urea nitrogen and 24 - hour urine protein excretion as compared with diabetic nephropathy group （ P 〈 0.05 ）. Conclusion： Vaspin may reduce the expression of NF - κB and TGF - β1 in renal tissue of diabetic nephropathy rats, delaying the progress of diabetic nephropathy.
出处
《中国中西医结合肾病杂志》
2016年第9期763-765,共3页
Chinese Journal of Integrated Traditional and Western Nephrology
基金
国家自然科学基金项目(No.81200591,81471025)
山西省留学回国人员科技活动择优资助项目(No.2013-251)
山西省回国留学人员科研基金资助项目(No.2015-109)
山西省国际科技合作计划项目(No.2015081029)
