Jhdm1d在小鼠胚胎发育中后期的表达及表观调控研究

Study of Expression and Epigenetic Regulation of Jhdm1d in Mouse Mid- later Gestation Period

本研究通过实时定量PCR(q RT-PCR)和免疫组织化学等技术,分析了Jhdm1d基因在小鼠胚胎发育中后期的表达模式。q RT-PCR结果显示,在胚胎发育中后期,Jhdm1d基因在各组织中的表达量明显不同,Jhdm1d在E12.5时期的脑和肝组织中表达最高,而在舌和肺组织中表达最低;在E15.5时期的肝脏组织中该基因的表达量最高,在肺和肾组织中的表达最低;在E18.5时期各组织中的表达均普遍较低。同时利用免疫组织化学的方法分析其在E15.5时期的表达,结果显示Jhdm1d在蛋白水平上的表达与定量结果基本相符。同时,亚硫酸氢盐测序的结果说明Jhdm1d的表达不受甲基化的影响。

In this study, we analyzed the expression patterns of Jhdmld in mouse mid-later gestation period in detail by quantitative real-time PCR （qRT-PCR）, and immunohistochemistry（IHC）, qRT-PCR results showed that in the mid-later gestation period, the expression of Jhdmld was significantly different in various tissues. At E12.5, Jhdmld was expressed abundantly in brain and liver, accompanied by the lowest level in tongue and lung. At E15.5 Jhdmld was expressed abundantly in liver, with the lowest level in lung and kidney. At E18.5, the expressions of Jhdmld in various tissues were almost very low. The immunohistochemistry results at E15.5 were consistent with qRT-PCR. The results of bisulfite sequencing demonstrated that Jhdmld expression was not regulated by DNA methylation.