沙利度胺对大鼠急性肺损伤的干预效果及作用机制探讨

Investigation of effect of thalidomide on the treatment of acute lung injury rats and it's mechanism

目的探讨沙利度胺对大鼠急性肺损伤的干预效果以及对TNF-α、IL-6等炎症因子的影响。方法按预防性给药及治疗性给药两种不同方法给予急性肺损伤大鼠沙利度胺,通过观察不同时间大鼠肺组织含水量、EB染料残留量、病理学改变等指标评估沙利度胺对于大鼠急性肺损伤的干预效果,同时测定大鼠血清TNF-α、IL-6等炎症因子浓度。结果治疗性给药可以降低大鼠肺组织的含水量,16 h时对肺泡毛细血管渗漏有改善作用。预防性给药可以在8h时降低肺组织含水量,在8、16 h时对肺泡毛细血管渗漏以及光镜下病理改变有改善作用。治疗性给药法可以降低8h时血清TNF-α浓度,也可以降低20 h时血清IL-6浓度;而预防性给药法可以降低8、16 h时血清TNF-α浓度,也可降低8、16、20 h时血清IL-6浓度。结论沙利度胺可以降低ALI大鼠模型肺毛细血管通透性,TNF-α与IL-6等炎症因子参与了该过程。

AIM To investigate the effect of thalidamide on the treatment of acute lung injury（ALI）ratsandthe associated irdlammatory factors such as TNF-α and IL-6. METHODS The ALI rats were given thalidomide in two differ- ent ways（treatment drug delivery and prevention drug delivery ）, and then the water content of lung, the residue of EB and the pathological change of lung were observed and compared with those in the model group in three different time points. The levels of TNF-α and IL-6 in rat serum were also determined. RESULTS In the treatment group, the water content of lung was decreased, and in 16 h the residue of EB was decreased. In the prevention group, the water content of lung was decreased in 8 h.The residues of EB were decreased in 8 and 16 h,the improvement in pathological changes was also observed. In the treatment group, the levels of TNF-α were decreased in 8 h, the levels of IL-6 were decreased in 20 h. In the prevention group, the levels of TNF-α were decreased in 8 and 16 h, the levels of IL-6 were decreased in 8,16 and 20 h. CONCLUSION Thalidamide can reduce the permeability of lung pulmonary capillary in ALI rats. TNF-α and IL-6 play important roles in the process.