HPLC法测定酵母菌细胞内维生素B_1含量

Determination of vitamin B_1 in yeast cells by HPLC

目的建立酵母菌细胞内维生素B_1的含量测定方法。方法采用Thermo DBS-C18分析柱（250 mm×4.6 mm,5μm）,流动相为水-甲醇（70∶30）,流速1.2 m L/min,柱温35℃,检测波长为238 nm。结果维生素B1在浓度1.0～5.0μg/m L浓度范围内线性关系良好（Y=294 400.5 X-65 837.9,r=0.997 9）,方法回收率为88%～92%,重现性在RSD=0.8%～1.24%（n=5）。结论本测定方法准确、可靠,重复性好,可用于测定酵母菌细胞内维生素B1含量。

Objective To establish the determination method of vitamin B_1 in yeast cells. Methods The analytical column was Thermo DBS-C18（ 250 mm × 4. 6 mm,5 μm） with a mobile phase consisting of water methanol（ 70∶30） as mobile phase at a flow rate of l. 2m L/min,the column temperature was 35 ℃ and UV detection was set at 238 nm. Results The linear range of thiamine was 1. 0-5. 0 μg/m L（ Y = 294 400. 5X-65 837. 9,r = 0. 997 9）,the method recovery rate rang was 88%-92% with RSD of 0. 8%-1. 24%（ n = 5）. Conclusion The method is accurate,reliable and repeatable which can be used to detect vitamin B1 in yeast cells.