心痛贴贴敷对大鼠心肌梗死影响的实验研究

The effect of Xintong sticker on myocardial infarction of rats

目的通过实验观察心痛贴贴敷对大鼠心肌梗死的影响。方法将正常Wistar大鼠60只按随机数字表法随机分为空白组、模型组、对照组和治疗组,每组15只。造模成功后对照组予硝酸甘油贴膜0.3mg心前区外贴,每次1贴,每日1次,每次维持24h,疗程10d。治疗组予心前区贴敷心痛贴1贴,每日1次,每次维持24h,疗程10d。免疫组织化学法测定丙二醛(MDA)、过氧化物歧化酶(SOD)、肿瘤坏死因子(TNF)-α、白细胞介素(IL)-6、B细胞淋巴瘤/白血病-2(Bcl-2)、Bax、胱天蛋白酶-3(caspase-3)、P物质(SP)、内皮细胞型一氧化氮合酶(eNOS)、诱导型一氧化氮合酶(iNOS)、钙敏感受体(CaSR)水平。结果 SOD:模型组、对照组、治疗组较空白组低(P<0.05),对照组和治疗组高于模型组(P<0.05),治疗组高于对照组,2组比较差异有统计学意义(P<0.05)。TNF-α:模型组、对照组较空白组高,对照组和治疗组低于模型组(P<0.05),治疗组低于对照组,2组比较差异有统计学意义(P<0.05)。caspase-3:模型组、对照组、治疗组高于空白组(P<0.05),治疗组低于模型组和对照组(P<0.05)。SP:模型组、对照组较空白组高,对照组和治疗组低于模型组(P<0.05),治疗组低于对照组,2组比较差异有统计学意义(P<0.05)。eNOS、iNOS、CaSR治疗组与对照组比较差异有统计学意义(P<0.05)。结论心痛贴减轻心肌梗死后心肌细胞坏死的机制与抑制脂质过氧化、促进SOD生成,减少MDA,抑制TNF-α、IL-6,减轻钙超载,上调eNOS的表达、下调iNOS表达有关。

Objective To observe the effect of Xintong sticker on myocardial infarction of rats.MethodsSixty healthy Wistar rats were randomly divided into control group,model group,blank control group and treatment group（n=15in each group）.After establishment of myocardial infarction model of rats,nitroglycerin stickers（0.3mg）were given to the control group for 24 hper day for 10 days,while XinTong stickers were used for24 hper day for 10 days in the treatment group.Then MDA,SOD,TNF-α,IL-6,Bcl-2,Bax,caspase-3,P substance,eNOS,iNOS,CaSR level was determined with immunohistochemistry.Results SOD level of the model group,the control group and the treatment group was lower（P 0.05）than that of the blank group,while SOD level of the treatment group and the control group was higher than that of the modeling group,the difference between two groups was statistically significant;TNF-αof the model group and the control group was higher compared with the blank group,while that of the control group and the treatment group was lower than the model group（P 0.05）,there was significant difference between two groups;caspase-3of the model group,control group and treatment group was higher than that of the control group（P 0.05）,while that of the treatment group was lower than the model group and the control group（P 0.05）.SP of the model group and the control group was higher than that of the control group,while that of the control and treatment group was lower than the model group（P 0.05）,the difference between the two groups was statistically significant;eNOS,iNOS and CaSR between treatment group and control group was also statistically significant（P 0.05）.Conclusion The mechanism of Xintong sticker in relieving myocardial necrosis after myocardial infarction was mainly in inhibiting lipid peroxidation,promoting SOD production,reducing MDA,inhibiting TNF-α,IL-6,alleviating calcium overload,increasing the expression of eNOS and down-regulating iNOS expression.