Nestin阳性细胞过表达DMP1对小鼠颌骨密度的影响

Influence of over expression of DMP1 in Nestin positive cells on the changes of jaw bone density in mice

目的探讨Nestin阳性细胞中牙本质基质蛋白1(DMP1)的作用。方法 DMP1是牙齿和骨中重要的矿化蛋白,但是Nestin阳性细胞中DMP1的作用仍不清楚。我们构建了Nestin阳性细胞中过表达DMP1的转基因小鼠。以野生型(wild type,WT)小鼠为对照组,通过HE染色,Micro CT检测DMP1-Tg小鼠颌骨的变化,进一步通过TRAP染色检测DMP1-Tg小鼠颌骨破骨方面的变化。结果相对于WT小鼠,DMP1-Tg小鼠颌骨骨量下降;TRAP染色显示DMP1-Tg小鼠下颌骨破骨细胞增多。结论在下颌骨发育过程中,Nestin阳性细胞中过表达DMP1对骨形成发挥抑制作用。

Objective To discuss the role of dental matrix protein 1（ DMP1） in Nestin（＋） cells. Methods DMP1 is an important mineralization protein of teeth and bones, but the role of DMP1 in Nestin（＋） cells is still not clear. A transgenic mouse model was con？structed in which DMP1 over expressed in Nestin（＋） cells. With wild mice as the control group, the DMP1-Tg mouse’ s jaw pheno？types by H＆amp;E staining and micro？CT were observed. Besides, the change of bone resorption in DMP1-Tg mouse by TRAP staining was also observed. Results Compared with WT mouse, the mandibular bone mass of DMP1-Tg mouse decreased;TRAP staining showed increased number of osteoclasts in DMP1-Tg mouse. Conclusion During the process of jaw development, the over expression of DMP1 in Nestin（＋） cell can inhibit osteogenesis.