人参皂苷对NLRP3炎性体激活的抑制作用

Inhibitory activities of ginsenosides on the activation of NLRP3 inflammasome

为筛选可抑制NLRP3炎性体激活的人参皂苷,在用ATP、尼日利亚菌素(nigericin)和二氧化硅(silica)等NLRP3炎性体诱导剂诱导的小鼠腹腔巨噬细胞(PMs)中分别用17种人参皂苷(PPT,PPD,Rb1,Rb2,Rb3,Rc,Rd,Re,Rg1,Rg2,Gg3,Rh1,Rh2,Ro,Rh2,Ro,c K,F1,F2)进行处理,通过对IL-1β的分泌量进行分析,明确具有抗炎作用的人参皂苷单体。在ATP诱导组中,IL-1β的分泌量在人参皂苷PPT、PPD和F1处理后下降最为明显(P<0.001);而在尼日利亚菌素诱导组中,除了PPT、PPD及F1外,Rg3对IL-1β的分泌也具有较高的抑制效果;在二氧化硅诱导组中,可抑制IL-1β的分泌的皂苷种类较多,包括PPT、PPD、Rd、Rg3、Rb3、Rb2、F2、Re、F1和Rh2(P<0.001)。本研究结果表明,PPT、PPD和F1对3种不同炎性体诱导剂引起的NLRP3炎性体激活均具有较强抑制作用(P<0.001)。

To screen ginsenosides with inhibitory activities on the activation of NLRP3 inflammasome, 17 ginsen- osides （ PPT, PPD, Rbl, Rb2, Rb3, Rc, Rd, Re, Rgl, Rg2, Gg3, Rhl, Rh2, Ro, Rh2, Ro, cK, F1, F2） were used to treat mouse peritoneal macrophages （PMs） induced with NLRP3 inflammasome inducers （ATP, nigericin, or silica）. In the present study, the higher anti-inflammatory activity of ginsenoside monomers were confirmed by analysis of IL-1β secretion in PMs. In the group of ATP induction, IL-1β secretion decreased after.ginsenoside treatments, the high inhibitory effect was found at treatments of PPT, PPD, and F1 （P 〈 0. 001）. At the group of the nigericin, Rg3 also possessed higher inhibitory effect on IL-1β secretion except PlYl＇, PPD, and F1 （P 〈 0. 001）. Furthermore, compared with groups of the ATP and nigericin, IL-1β secretion at the group of silica decreased after treatments of various ginsenoside monomers, including PPT, PPD, Rd, Rg3, Rb3, Rb2, F2, Re, F1, and Rh2 （P 〈0. 001）. The present study indicates that PPT, PPD, and F1 can efficiently inhibit NLRP3 inflam- masome activation induced by different inducers.