L9（34）正交试验优化柑橘中橙皮苷的提取工艺

Extraction Process of Hesperidin in Citrus Optimized by L9 （34） Orthogonal Test

[目的]开展柑橘皮中橙皮苷提取纯化工艺研究，充分利用汉中丰富的柑橘资源，提高其经济附加值。[方法]以汉中柑橘皮为研究材料，采用碱浸酸析法提取橙皮苷，以碱的种类、碱添加量、提取温度、提取时间、料液比进行单因素试验，以碱添加量、提取温度、提取时间、料液比进行L9（34）正交试验优化橙皮苷的提取工艺，以超高效液相色谱检测验证优化结果。[结果]影响柑橘皮中橙皮苷提取纯化工艺因素大小依次为提取时间、碱添加量、提取温度、料液比；1 g柑橘皮添加0．08 g的Ca（OH）2，提取时间0．5 h，温度30℃，料液比1∶30 g／mL的工艺条件下能得到最佳提取效果，粗产品收率为4．5％。[结论]试验所得优化参数和提取工艺稳定，选用的设备操作简便、快速，具有良好的应用前景。

[Objective] In order to carry out the research on extraction and purification process of hesperidin in citrus peel, make full use of abundant citrus resources in Hanzhong and increase the added economical value.[Method] Taking the Hanzhong citrus peel as research mate-rial, hesperidin was extracted by alkali extraction and acid precipitation method.The single factor experiment was carried out with the kinds of alkali, alkali content, extraction temperature, extraction time and the ratio of material to liquid.Alkali dosage, extraction temperature, ex-traction time and solid-liquid ratio were studied by L9 （34 ） orthogonal test to optimize the extraction technology of hesperidin.The ultra per-formance liquid chromatography was used to test the optimization results .[ Result] The sequence for the factors affecting extraction and purifi-cation process of hesperidin in citrus peel was extraction time, alkali weight, extraction temperature and the ratio of material to liquid.When the process conditions were 0.08 g of Ca（OH）2 per gram citrus peel, extraction time 0.5 h, temperature 30 ℃, and the material and liquid ratio 1∶30 g/mL, the best extraction effect could be obtained.The yield rate of crude product was 4.5%.[Conclusion] The optimization pa-rameters and extraction process obtained in the test were stable, and the operation of selected equipment was simple and rapid, which had good application prospects.