短叶对齿藓组织培养的研究

Tissue Culture of Didymodon tectorum

以采自白云鄂博主矿区的短叶对齿藓为试验材料,研究了不同消毒剂以及不同浓度植物激素6-BA和IAA对短叶对齿藓愈伤组织诱导和分化的影响。结果表明:短叶对齿藓配子体最佳消毒剂及作用时间为体积浓度百分比75%乙醇浸泡30s,再用质量浓度0.1g/L升汞消毒90s;采用Knop培养基培养短叶对齿藓茎叶段,质量浓度为0.1mg/L 6-BA促进愈伤组织分化形成配子体,质量浓度1.0mg/L 6-BA则抑制短叶对齿藓愈伤组织形成,IAA有助于原丝体的萌发生长。

With Didymodon tectorum collected from the main mine of Baiyun obo as experimental material,we studied the effect of different disinfectants and different concentrations of plant hormone 6-BA and IAA,respectively on callus induction and differentiation.It showed that the best disinfectant for D.tectorumgametophyte is 75% alcohol immersion for 30 sand 0.1g/L mercuric chloride for 90 s.Using Knop medium to culture the upper stem-leaf segments of D.tectorum,and a low concentration of 6-BA（0.1mg/L）promotes callus differentiation and gametophyte formation.However,high concentration of 6-BA（1.0mg/L）restrains the callus formation.IAA helps protonemata germinate.