Calpain抑制剂对肝癌细胞MHCC97-H中Calpain-2蛋白表达的影响

Effect of Calpain Inhibitors of Different Concentration on Expression of Calpain-2 Protein in Hepatocellular Carcinoma Cell Line MHCC97-H

目的:观察4种Calpain抑制剂不同浓度及作用时间对肝癌细胞MHCC97-H中Calpain-2蛋白表达的影响。方法:实验分为对照组、DMSO组及4种抑制剂组,4种抑制剂组分别给予高、中、低浓度处理肝癌MHCC97-H细胞24、48及72 h;用Western blot方法检测MHCC97-H中Calpain-2蛋白的表达,确定4种抑制剂的最适浓度和作用时间。结果:与对照组、DMSO组、其他时段各浓度组及同时段其他浓度组比较,高浓度ALM作用72 h后肝癌细胞MHCC97-H中Calpain-2蛋白的表达下降(P<0.05);与对照组、DMSO组、其他时段各浓度组及同时段其他浓度组比较,高浓度Calpain inhibitor IV、Calpain inhibitor VI、Calpastatin抑制剂72 h组肝癌细胞MHCC97-H中Calpain-2蛋白的表达量显著下降(P<0.01)。结论:4种高浓度Calpain抑制剂处理肝癌细胞MHCC97-H 72 h后可抑制肝癌细胞MHCC97-H中Calpain-2蛋白的表达。

Objective： To observe the effect of different concentration of Calpain inhibitor and different time on the expression of Calpain-2 protein in hepatocellular carcinoma cell line MHCC97-H.Methods： There were control group,DMSO group and 4 inhibiting group in the experiment. In the inhibiting groups,hepatocellular carcinoma MHCC97-H cells were treated with high,middle and low concentration of 4 different Calpain inhibitors for 24 h,48 h and 72 h,respectively. The expression of calpain-2 protein in MHCC97-H cells was detected by Western blot to determine the optimum concentration and time for the four inhibitors. Results： Compared with the control group,DMSO group,other groups of different concentration and different inhibiting time,the expression of Calpain-2 protein in HCC cell line MHCC97-H was significantly decreased after high concentration of ALLM inhibiting for72 h（ P 〈0. 05）. Compared with the control group,DMSO group,other groups of different concentration and different inhibiting time,the expression of Calpain-2 protein in HCC cell line MHCC97-H was significantly decreased after high concentration of Calpain inhibitor IV,Calpain inhibitor VI,and Calpastatin inhibitor inhibiting for 72 h（ P 〈0. 01）. Conclusion： The expression of Calpain-2 protein in hepatocellular carcinoma cell line MHCC97-H can be inhibited by 4 high concentration of Calpain inhibitors in the treatment of hepatocellular carcinoma cell line MHCC97-H 72 h.