联合诱导法对大鼠肝微粒体细胞色素P450酶系的影响

Effects of the Combined Induction of Phenobarbital and β-naphthoflavone on Liver Microsomal Cytochrome P450 in Rats

目的:观察苯巴比妥钠(PB)与β-萘黄酮(β-NF)联合应用对大鼠肝微粒体细胞色素P450(CYP450)酶系的影响。方法:16只SD雄性大鼠随机分为实验组和对照组,实验组腹腔注射PB和β-NF,对照组给予等量生理盐水,连续4d后取各组大鼠肝脏组织制备肝微粒体;测定肝脏脏器系数,采用Lowry法测定肝微粒体蛋白含量,分光光度法检测CYP450、细胞色素b5(Cytb5)含量和CYP3A活性,荧光分光光度法测定CYP1A2活性。结果:PB与β-NF联合应用后,大鼠肝脏脏器系数,肝微粒体蛋白含量、CYP450、Cytb5含量及CYP1A2、CYP3A活性较对照组均有增加,差异有统计学意义(P<0.05)。结论:PB和β-NF腹腔注射对大鼠肝微粒体CYP450酶系有较强的诱导效应。

Objective： To observe the effects of combined induction of phenobarbital and β-naphthoflavone on liver microsomal cytochrome P450 in rats. Methods： 16 male SD rats were randomly divided into 2 groups,the experimental group in which each animal was intraperitoneally injected with phenobarbital and β-naphthoflavone,and the control group in which each animal received equal volume of saline. After this treatment for 4 days successively,the liver microsomes were prepared and liver / body weight ratios were detected. The contents of protein in liver microsomes were measured by Lowry method. Spectrophotometric was adopted to determine the contents of CYP450,cytochrome b5（ cytb5） content and CYP3 A activity,and the CYP1A2 activity was determined by fluorescence spectrophotometry. Results： After combined induction of phenobarbital and β-naphthoflavone,the liver organ coefficient,liver microsomal protein content,CYP450,cytb5 content and CYP3 A activity in the rat liver were increased compared with the control group,and the difference was statistically significant（ P 〈0. 05）. Conclusion： The combination of phenobarbital and β-naphthoflavone has a strong induction effect to CYP450 of liver microsomes in rats.