失功能高密度脂蛋白对冠心病患者血管新生的影响

Effect of dysfunctional high density lipoprotein on angiogenesis in patients with coronary atherosclerotic heart disease

目的研究冠状动脉粥样硬化性心脏病患者体内高密度脂蛋白对内皮细胞管状形成的影响。方法采用超高速梯度离心法提取正常志愿者(对照组)和冠状动脉粥样硬化性心脏病患者(实验组)体内的高密度脂蛋白,然后通过cell-free法检测两组高密度脂蛋白抗氧化能力的差异(用相对荧光强度RFU表示),最后用Matrix gel法检测两组高密度脂蛋白对内皮细胞管状形成的影响(用血管条长度表示)。结果与对照组比较,实验组高密度脂蛋白的抗氧化能力明显降低(369.65±68.38 vs 1 017.60±116.52,P<0.05);对照组高密度脂蛋白促进内皮细胞管状形成,实验组高密度脂蛋白可抑制内皮细胞管状形成;与对照组比较,实验组血管条长度变小(P<0.05)。结论冠状动脉粥样硬化性心脏病患者体内的失功能高密度脂蛋白可抑制内皮细胞管状形成。

Objective To explore the effect of high density lipoprotein（ HDL） on the tube formation in patients with coronary atherosclerotic heart disease（ CAHD）. Methods Ultra high speed gradient centrifugation was used to centrifuge HDL from normal volunteers（ control group,n = 20） and CAHD patients（ CAHD group,n = 20）. Then the difference of antioxidant capacity of HDL between two groups was detected by cell-free assay. The gel matrix assay was applied to detect the endothelial cell tube formation. Results Compared with control group,antioxidant capacity of HDL in CAHD group was significantly reduced（ 369. 65 ± 68. 38 vs 1 017. 60 ±116. 52,P〈0. 05）. The endothelial cell tube formation was increased by HDL in control group but decreased in CAHD group. The length of vessel was shorter in CAHD group than in control group（ P〈0. 05）. Conclusion The dysfunctional HDL in CAHD patients may inhibit the endothelial cell tube formation,which may partly explain why simple elevation of HDL levels can not improve the prognosis of patients with CAHD.