乳化异氟醚预处理对肺缺血再灌注损伤后核转录因子κB表达的影响

Effect of emulsified isoflurane pretreatment on NF-κB expression in lung ischemia/reperfusion injury of rats

目的：研究乳化异氟醚（EI）预处理对大鼠肺缺血再灌注肺损伤的保护机制。方法：雄性Wistar大鼠随机分为对照组、模型组、脂肪乳组和EI组。除对照组外，其余3组均参照改良的Eppinger方法建立大鼠肺缺血再灌注损伤模型，脂肪乳组和EI组在建模前分别作脂肪乳和EI预处理。评价各组大鼠肺组织病理形态变化；测定肺组织髓过氧化物酸（MPO）、肿瘤坏死因子-α（TNF-α）水平；免疫组织化学和免疫印迹检测各组大鼠肺组织中核转录因子κB（NF-κB）的表达。结果：肺缺血再灌注2h后，模型组肺组织MPO、TNF-α含量升高，高于对照组及乳化异氟醚组，有统计学意义；模型组NF-κB表达量较高，与脂肪乳组无差异，但与其余2组差异均具有统计学意义；EI组与对照组比较，差异无统计学意义。结论：乳化异氟醚预处理可减轻肺缺血再灌注损伤，其机制可能是通过抑制NF-κB信号通路发挥作用。

Objective： To investigate the protective mechanism of emulsified isoflurane pretreatment on the lung ischemia/ reperfusion injury in rats. Methods： Male Wistar rats were randomly divided into control group, model group, lipid emulsion group（LE group） and emulsified isoflurane group（El group）. Except for the control group, the remaining 3 groups were established by the modified Eppinger method; LE group and EI group were pretreated with lipid emulsion and emulsified isoflurane before modeling. Lung tissues were restored to analyze the pathological changes; The level of lung tissue myeloperoxidase（MPO） and TNF-α were determined; The expression of NF-κB in the lung tissues was tested by immunohistochemical staining and Western blotting. Results： Two hours after reperfusion, the MPO and TNF-α levels of model group were significantly higher than the control group and EI group. There was significantly higher expression of NF- κB in the model group than the other 3 groups, but no significant difference was found between the model group and the LE group. There was no significant difference in the NF-κB expression level between the control group and the EI group. Conclusion： The emulsified isoflurane pretreatment can protect the lung against ischemia/reperfusion injury by inhibiting the activation of NF-κB signal pathway.