一株锶高效富集真菌的筛选、鉴定及富集特性

A Research on the Screening,Identification,and Enrichment Characteristics of a Fungi Strain with Efficient Enrichment to Strontium

为利用微生物技术修复环境中的锶(Sr)污染,通过从锶污染的土壤中分离、筛选Sr的高效富集菌种,采用响应曲面法优化该菌对Sr的富集条件.结果显示,通过富集培养、稀释平板涂布筛选出一株对Sr具有较强富集能力的真菌,经鉴定该菌为Talaromyces sp..利用响应曲面法优化后,各因素的最佳水平组合为:培养温度35℃,p H值3.0,Sr初始质量浓度50 mg/L,该菌(干重)对Sr的富集量达到1 815.21 mg/kg,优化后的富集量与理论预测值较接近,说明响应曲面法建立的模型可用于预测该菌对Sr2+的富集特性.结果表明,筛选得到的Talaromyces sp.对Sr的富集能力强,可用于环境中的Sr污染修复.

In order to use microorganism removing environment of strontium, this paper obtained an efficient enrichment of fungi strain by screening of microorganisms from Sr^2＋ polluted soil. Meanwhile, Sr enrichment conditions of the fungi strain was optimized by using response surface method. The results showed a fungi strain has obtained, which has efficient enrichment ability for St, identifying as Talaromyces sp. . After the optimizing by response surface method, the best combination of various factors was： a culture tempera- ture of 35℃, a pH value of 3 and a initial concentration of 50 mg/L, the enrichment value was up to 1815.21 mg/kg, which was closed to the prediction. It showed that the model established from response surface method can be used to predict the fungus enrich- ment characteristics of Sr^2＋. In general, Talaromyees sp. had stronger capacity of enriching St, it should have a great application po- tential to remove environment of strontium.