结直肠腺瘤及腺癌组织中FOXO1表达及其临床意义

Expression and clinical significance of FOXO1 in colorectal adenoma and adenocarcinoma

目的检测结直肠腺瘤及腺癌组织中转录因子FOXO1的表达,在组织学水平上初步探讨FOXO1在结直肠腺瘤癌变过程中的作用及其与临床病理特征的关系。方法采用Western blot法免疫组化SP两步法检测146例结直肠腺瘤、48例结直肠腺癌及48例癌旁组织中FOXO1蛋白的表达水平。结果 FOXO1在结直肠腺瘤及腺癌组织中的表达明显低于正常组织,组间比较差异有统计学意义(P<0.01)。结直肠腺瘤组织中FOXO1表达与肿物大小相关,腺癌组织中FOXO1表达与肿瘤分化程度相关。结论 FOXO1在人类肠道癌前病变(腺瘤)及腺癌组织细胞核表达减少和缺失,可能是导致结肠腺癌发病的机制之一,其在结直肠癌的发生、发展过程中起抑癌基因的作用,有望成为下一个潜在的临床治疗靶点。

Purpose To investigate the expression level of transcriptional factor FOXO1 in colorectal adenoma and adenocarcinoma, and to explore its relationship with clinicopathological characteristics. Methods The protion of FOXO1 was detected by immnnohisto- chemistry of SP two-step and Western blot in 146 cases of colorectal adenoma, 48 cases of colorectal adenocarcinoma and 48 cases of the adjacent mucosa. Results The expression of FOXO1 in adenoma and adenocarcinoma was significantly lower than that in normal tissues, and the differences between the groups were statistically significant （P 〈 0. 01 ）. The expression level of FOXO1 proteins was related to the size of the tumors in colorectal adenoma and the pathological grade of cancer tissues in colorectal adenocarcinoma. Con- dusion The findings of our study indicate that the decreased or deleted expression of FOXO1 in colorectal precancerous lesions （ ade- noma） and adenocarcinoma may be one of the reasons to cause colorectal adenocarcinoma. It acts as tumor suppressor gene and plays a vital role in the carcinogenesis and development of colorectal cancer. FOXO1 seems to become the next potential target of clinical treatment.