摘要
目的了解2015年四川省风疹病毒的基因特征。方法采用Vero/SLAM细胞分离培养风疹病毒，通过逆转录-聚合酶链反应（Reverse Transcription-Polymerase Chain Reaction，RT-PCR）方法扩增风疹病毒E1基因编码的739个核苷酸片段，并对扩增产物进行序列测定和分析。结果19株风疹病毒分离株均属于2B基因型，分离株之间的核苷酸和氨基酸同源性分别为99%-100%和97.9%-100%，重要位点未发生变异。结论2015年四川省流行的风疹病毒为2B基因型。
Objective To understand the genetic characteristics of rubella virus in Sichuan province in 2015. Methods Vero/SLAM cells were used for rubella virus isolation and culture. The 739 nucleotides of E1 gene was amplified by Reverse Transcription-Polymerase Chain Reaction （RT-PCR） and subjected to sequence and homological analysis. Results 19 strains of rubella virus were isolated and identified as genotype 2B. The homology of nucleotides and amino acids were 99%-100% and 97.9%- 100% , respectively. There were no changes in the important antigenic epitopes. Conclusions The rubella virus genotype circulated in Sichuan province in 2015 was genotype 2B.
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
2016年第5期425-429,共5页
Chinese Journal of Experimental and Clinical Virology
