2011—2013年无锡地区人腺病毒流行特征与分型

Epidemiological characteristic and typing of human adenovirus in Wuxi area of 2011-2013

目的了解2011-2013年无锡地区人腺病毒的流行的基因型及规律。方法对2011-2013年无锡市人民医院送检的1 893份急性呼吸道感染标本，用Real-time PCR方法扩增腺病毒基因，检出的阳性标本接种Hep-2细胞分离病毒，然后对其扩增产物进行纯化和测序比对，所得基因序列用Bioedit和MEGA 5.0进行进化树分析。 结果2011-2013年无锡地区人腺病毒全年都有出现，多发生在冬春季，1-3岁的儿童检出率（44.26%）最高，型别主要有B亚群的HAdV-3型4株（13.33%）、HAdV-7型6株（20.00%）和C亚群HAdV-1型5株（16.66%）、HAdV-2型8株（26.67%）、HAdV-5型3株（10.00%）、HAdV-6型4株（13.33%）。基因进化树形成2个独立的分支，C亚群逐渐成为优势株。结论2011-2013年无锡地区人腺病毒有明显的季节性，多发生在冬春季，1-3岁的儿童是主要的易感人群。B和C两种亚群6个血清型为优势流行株，推测有B亚群向C亚群转变的流行趋势。

Objective To study the epidemiological characteristics and type of human adenovirus （HAdv） in Wuxi area in 2011-2013. Methods A total of 1983 acute respiratory infections samples were collected from Wuxi People＇s Hospital during 2011-2013. HAdv was detected by Real-time PCR, the positive samples were inoculated into Hep-2 cells to isolate the viruses. The amplificons were purified and sequenced, and the sequences were analyzed by Bioedit and MEGA 5.0. Results Human adenovirus was appeared throughout the year in Wuxi area during 2011-2013. In winter and spring, the highest rate （44.26%） was detected in 1-3 years old children. There were 4 strains （ 13.33% ） for HAdV-3, 6 strains for HAdV-7（20.00% ） of B subgroup,5 strains （ 16.66% ） for HAdV-1, 8 strains （26.67%） for HAdV-2, 3 strains （10.00%） for HAdV-5, 4 strains （13.33%） for HAdV-6 of C subgroup. The phylogenetic tree formed two independent branches and C subgroup gradually became advantage. Conclusions Human adenovirus had obvious seasonal characteristics in Wuxi area during 2011- 2013, mainly in winter and spring ; 1-3 years old children were the main susceptible population. Six types of B and C subgroups were the dominant epidemic strains, with the epidemic tendency transformed from subgroup B to C.