重组腺病毒载体HIV-1 env疫苗合用佐剂的免疫效果研究

Adjuvants enhance cellular immune response induced by recombinant adenoviral vector encoding HIV-1 env gene in mice

目的探讨添加IL-7、IL-21基因佐剂及Poly（I：C）、CpG ODN对艾滋病疫苗的免疫效果有无增强作用。方法构建了表达IL-7、IL-21基因的质粒pVR-IL7、pVR-IL21，以DNA疫苗（pVR-HIVenv）对小鼠进行初免，以腺病毒载体疫苗（Ad5-HIVenv）添加佐剂进行加强免疫，末次免疫后两周分别采用酶联免疫斑点法（ELISPOT）和酶联免疫吸附法（ELISA）检测免疫小鼠诱导的特异性细胞免疫反应和体液免疫反应。结果ELISPOT结果显示Ad5-HIVenv疫苗联合IL7组小鼠每百万个脾淋巴细胞中分泌IFN-γ的细胞数（SFC）/106高于疫苗单独免疫组小鼠（P〈0.05）；IL-7和CpG ODN作为佐剂同时使用可以进一步的提高免疫反应（P〈0.05）。结论Ad5-HIVenv疫苗联合IL7佐剂及CpG ODN具有较强的细胞免疫反应，而联用IL21佐剂则未见明显的免疫增强作用。

Objective To study the immune effects of IL-7, IL-21 as gene adjuvants, Poly（I：C） and CpG ODN for HIV vaccine. Methods Mouse interleukin 7 DNA adjuvant （pVR-IL7） and mouse interleukin 21 DNA adjuvant （pVR-IL21） were constructed. BALB/c mice received DNA prime-adenoviral vector boost immunization with pVR-HIVenv-Ad5-HIVenv alone or combined with pVR-IL7 ,pVR-IL21, Poly （I：C） and CpG ODN. Cellular and humoral immune responses were assessed by IFN-g enzyme-linked immunosorbent spot assay and enzyme-linked immunosorbent assay. Results Compared with those immunized with vaccines alone, the mice immunized with pVR-IL7 had increased specific cellular response （P 〈 0.05 ） , CpG ODN had syncrgic effects with IL7 as adjuvants （ P 〈 0. 05 ） Conclusion IL7 but not IL21 can enhance the specific cellular immune response of Ad5-HIVenv in mice.