Sanger法测定季节性H3N2亚型流感病毒全基因组序列

Sequencing of seasonal influenza A （H3N2） virus

目的对流行的季节性甲型H3N2亚型流感病毒进行全基因组测序。方法本文对甲型H3N2亚型流感病毒测序过程中的PCR扩增引物进行优化及精简，同时对PCR产物纯化方法进行了改进。使用优化后的34对PCR引物和优化后的纯化方法，对194株甲型H3N2亚型病毒进行了测序。结果利用优化后的测序方法，可保证获得甲型H3N2亚型流感病毒的8个片段全基因组序列，而且大大缩短了实验时间，节约了实验成本和人力。结论我们应大力开展A（H3N2）亚型流感病毒的序列测定工作，及时发现病毒变异情况，以推选出与人群中的流行株更为匹配的疫苗株。

Objective To sequencing the whole genome of seasonal influenza A（H3N2） viruses. Methods Optimized and reduced the PCR primers of A （H3N2） virus, and improved the purification method of PCR production. Use this method, 194 A（H3N2） viruses were sequenced. Results Optimized sequencing method was used for full genomc sequencing of influenza A （H3N2） virus, 8 segments of all A （H3N2） viruses were obtained. This optimized method reduced the time required for sequencing, and saved experimental cost. Conclusions We should carry out the sequencing analysis of influenza A （H3N2） viruses, to timely identify the variation of viruses, and to select more appropriate vaccine strains for human population.