GC-MS法分析“发汗”对续断挥发性成分的影响

Effect of “Fahan” on volatile constituents in Dipsaci Radix by GC-MS

目的通过GC-MS法分析＂发汗＂对续断Dipsacus asper Wall.ex Henry挥发性成分的影响。方法水蒸气蒸馏法提取续断乙醚萃取物挥发油后,GC-MS法鉴定其成分,面积归一化法计算各成分相对含有量。结果续断在＂发汗＂前后均鉴定出28种成分,其中26种为共有成分。＂发汗＂前未检测出9,12,15-十八碳三烯酸甲酯和9-亚甲基-9H-芴,而＂发汗＂后未检测出顺-13-十八碳烯酸和十七烷醇。结论 ＂发汗＂后续断中共有成分的含有量均有明显变化,变化范围为30.81%-99.34%。

AIM To analyze the effect of ＂Fahan＂（ herbs piled and naturally dried） on volatile constituents in Dipsacus asper Wall. ex Henry by GC-MS. METHODS After the volatile oil in D. asper ethereal extract was extracted by steam distillation,its constituents were identified by GC-MS,then the relative contents of various constituents were calculated by area normalization method. RESULTS Before and after ＂Fahan＂,twenty-eight constituents were identified in D. asper,twenty-six of which were common components. Methyl 9,12,15-octadecatrienoate and 9-methylene-9H-fluorene were not detected before ＂Fahan＂,while 11-octadecenoicacid,（ 11Z）-and 1-heptadecanol were not detected after ＂Fahan＂. CONCLUSION After ＂Fahan＂,the contents of common components in D. asper all show obvious changes within the ranges of 30. 81%- 99. 34%.