一株铜绿微囊藻溶藻菌的分离鉴定和溶藻特性

Isolation and identification of an algae-lysing bacterium and its algicidal characteristics against Microcystis aeruginosa

以铜绿微囊藻为溶解对象,从富营养化水体中分离到1株溶藻菌H1,通过形态特征及16S rDNA序列分析,该菌株属寡养单胞菌属(Stenotrophomonas sp.),GenBank登录号为KU359254。探讨了菌株H1对铜绿微囊藻(FACHB-1326)的溶藻方式,菌藻浓度、菌的生长时期及光照、pH、温度等环境因子对铜绿微囊藻溶藻效果的影响。结果表明,菌株H1溶藻方式主要是间接作用溶藻。处于对数期的菌株H1抑藻效果最高,溶藻率达77.9%;菌液浓度达10~8 CFU·mL^(-1)以上,溶藻率最高,为81.8%;在pH7、30℃条件下,活性较强;pH7时30℃条件下,溶藻率为72.5%,63.2%;菌株H1对微囊藻生长的抑制效果是全黑条件>光循环条件>全光条件,全黑条件下溶藻率最高为59.9%。

An algicidal bacterium strain named as H1 with algicidal activity against the toxic algae microcystis aeruginosa was isolated from the＇eutrophication of water bodies. Based on the morphological characteristics and 16S rDNA gene sequence analysis, the strain H1 belongs to Stenotrophomonas sp. （GenBank number： KU359254）. The algicidal mode of algae-lysis bacterium H1, the bacterium concentration, and the effects of the bacterium growth, light, pH and temperature on algae-lysing of microcystis aeruginosa were studied. The result showed that the algi- cidal mode of the strain H1 was in an indirect way, and the strain H1 in logarithmic phase could dissolve microcystis aeruginosa with a removal rate of 77.9%. The sterile condensed bacteria-free filtrated with the concentration more than 10^8CFU·mL^-1 showed the highest removal rate of 81.8%. Under the condition of pH7 and 30℃, the removal rate was 72.5% and 63.2%, respectively. The inhibition effect of the strain H1 on microcystis aeruginosa was： full darkness〉 12L/12D〉full light, with the highest rate of 59.9 % in the full darkness condition.