间歇低氧模型大鼠肝脏组织中IGF-1、IGFBP-3的表达及分析

Analysis of IGF-1 and IGFBP-3 level in liver tissue of rat with intermittent hypoxia

目的检测阻塞性睡眠呼吸暂停低通气综合征(OSAHS)间歇性低氧模型大鼠肝脏组织中IGF-I及IGFBP-3水平的变化,探讨间歇低氧对肝脏的影响,为研究儿童OSAHS的病理生理改变提供客观依据。方法 24只雌性20日龄SD大鼠随机分为对照组、轻度低氧组、重度低氧组,轻度低氧组、重度低氧组大鼠饲养于间歇性缺氧密闭箱内8 h/d。35 d后处死取材,免疫组织化学法检测肝脏IGF-1、IGFBP-3。结果对照组、轻度低氧组、重度低氧组肝组织IGF-1的阳性表达分别为2/8、3/8和6/8,IGFBP-3的阳性表达分别为3/8、4/8和7/8,有逐渐升高趋势。对照组与轻度低氧组肝组织IGF-1、IGFBP-3的表达差异无统计学意义。对照组与重度低氧组肝组织IGF-1、IGFBP-3的表达差异无统计学意义。Spearman秩相关检验表明,重度低氧组中IGF-1与IGFBP-3的高表达呈正相关(r=0.746,P<0.05)。结论间歇性低氧模型中大鼠肝细胞IGF-1与IGFBP-3含量受生长激素(GH)-IGF1-IGFBP3轴功能紊乱的影响不大,重度低氧组大鼠肝细胞IGF-1与IGFBP-3含量与对照组及轻度低氧组比较无显著差异。

Objective To detect the insulin-like growth factor 1（IGF-1）and insulin-like growth factors binding protein 3（IGFBP-3）levels in liver tissue of rat with intermittent hypoxia,and to investigate theeffect of intermittent hypoxia on liver,in order to provide pathophysiologic evidence of obstructive sleep apnea-hypopnea syndrome （OSAHS）in chil-dren.Methods A total of 20 day-old Sprague-Dawley female rats were randomly divided into 3 groups：mild hypoxia group,severe hypoxia group and control group,the first two groups were raised in sealed hypoxia box 8-hour per day and were sacrificed 35 days after,The level of IGF-1 and IGFBP-3 of liver tissure was detected by immunohistochemi-cal method.Results The expression of IGF-1 and IGFBP-3 of liver in control group,mild hypoxia group and severe hypoxia group increased gradually.The positiveexpression rate of IGF-1 were 2 /8,3 /8 and 6 /8 in control group,low oxygen group and severe hypoxia group,respectively.The positiveexpression rate of IGFBP-3 were 3 /8、4 /8 and 7 /8 in there group respectively.There was no significant difference in level of IGF-1 and IGFBP-3 between the control group and the mild hypoxia（χ2 =0.157,P =0.315）,and between the control group and thesevere hypoxia （χ2 =0.066,P =0.132）.Spearman-test showed a positive correlation between level of IGF-1 and IGFBP-3 in severe hypoxia group （r =0.746,P 〈0.05）.Conclusion GH-IGF1-IGFBP3axisdysfunctionhas little effect on IGF-1and IGFBP-3 in liver tissue of rate withintermittent hypoxia .There was no significant difference in level of IGF-1 and IGFBP-3 among three groups.