越橘花果抗氧化物代谢通路及花青素合成酶基因表达分析

Analysis of Antioxidants Metabolic Pathway and Expression of Anthocyanin Biosynthetic Genes in Blueberry Flower and Fruit

以越橘花、幼果、成熟果为试材,基于转录组测序开展了抗氧化物代谢通路和花青素生物合成酶基因表达研究。结果表明,抗氧化物代谢通路中,黄酮类、苯丙素类合成通路是活跃的,叶酸类、类胡萝卜素、二苯乙烯类、二芳基庚酸类、姜酚、二萜类化合物的合成通路是部分支路活跃的,而其他抗氧化物通路是不活跃的。花青素合成酶基因中,苯丙氨酸裂解酶、肉桂酸羟化酶、查尔酮合成酶、4-羟查尔酮异构酶、黄烷酮-3-羟化酶、二氢黄酮醇-4-还原酶、花青素合成酶、类黄酮3,5-糖苷转移酶是幼果或成熟果中表达上调的。

Studies on metabolism pathway of antioxidants and expression of anthocyanin biosyn- thetic genes are carried out by using transcriptome sequencing technology to blueberry flower, young fruit and ripe fruit. The results show that, among antioxidant metabolic pathways, the biosynthesises of flavonoids and phenylpropanoid are active, and the biosynthesises of folate, ca- rotenoid, stilbenoid, diarylheptanoid, gingerol, and diterpenoid are partly active, while the other antioxidant pathways are inactive. They also show that,among the enzymes related to anthocy- anin biosynthesis, phenylalanine ammonia-lyase, cinnamate 4-hydroxylase, chalcone synthase, chalcone isomerase, flavanone 3-hydroxylase, dihydroflavanol 4-reductase, anthocyanidin syn- thase,and anthocyanidin/flavonol 3-O-glucosyltransferase are up-regulated in young fruit or ripe fruit, compared with flower.