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AhHDA1异源表达影响拟南芥植株干旱性 被引量:9

Heterologous Expression of AhHDA1 Affects Drought Resistance of Arabidopsis Plants
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摘要 将花生组蛋白去乙酰化酶1基因(Arachis hygogaea histone deacetylase 1,AhHDA1)转化拟南芥野生型Col-0和突变体had6,对获得的纯合转基因植株进行抗旱性分析,并对ABA合成及相关响应基因表达进行检测.结果表明:AhHDA1蛋白定位于细胞核,在干旱条件下,与hda6突变体比较,p35S%HDA1/hda6拟南芥植株的叶片相对含水量降低,气孔开度增大,干旱存活率降低,基本回复了Col的表型;体内ABA合成关键酶基因At NCED3和ABA信号途径重要转录因子At ABF3基因的表达降低,但RD29A基因表达提高.而p35S%HDA1/Col较p35S%HDA1/hda6和Col的抗旱性关键生理指标降低更加显著.说明hda6突变体表型回复确实由于外源AhHDA1的表达引起的,推测AhHDA1影响植物抗旱性与ABA的合成与信号通路相关. AhHDA1( Arachis hygogaea histone deacetylase 1) was transformed into Arabidopsis Col-0 and hda6 mutants. The drought resistance of the homozygous transgenic plants,the expression of ABA biosynthesis and droughtresponse genes were analyzed. The results showed as follows: AhHDA1 protein was located in the nucleus; compared with hda6 mutants,both the relative water content and survival rates of leaves of p35S% HDA1 / hda6 plants under drought were lower,and the stomatal apertures were higher under drought treatment,which nearly recovered Col phenotype. The expression of the key enzyme gene At NCED3 in ABA biosynthesis and the key transcription factor At ABF3 in ABA signal pathway in the p35S% HDA1 / hda6 plant was found significantly higher than those of hda6,whereas the expression of RD29 A was lower than that of hda6. The key physical parameters mediated differential drought resistance of p35S% HDA1 / Col,p35S% HDA1 / hda6 and Col. It indicated that transformed AhHDA1 recovered hda6 mutants phenotype,which might affect drought response by ABA biosynthesis and signaling.
出处 《华南师范大学学报(自然科学版)》 CAS 北大核心 2016年第5期52-57,共6页 Journal of South China Normal University(Natural Science Edition)
基金 国家自然科学基金项目(31471422)
关键词 拟南芥 hda6突变体 AhHDA1 干旱胁迫 Arabidopsis thaliana hda6 mutants AhHDA1 drought stress
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