南美天胡荽无菌快速繁殖技术研究

Sterile Rapid Propagation Technique of Hydrocotyle vulgaris L.

旨在研究不同培养条件对南美天胡荽不定芽再生和生根状况的影响并筛选出最适培养条件。以南美天胡荽带腋芽茎段(约1.0 cm)为外植体,进行腋芽诱导、丛生芽增殖及植株再生研究。结果表明生长调节剂种类及质量浓度对南美天胡荽不定芽的出芽时间和再生率以及生根率有明显影响。所做结果分化率高达89%,生根率高达98%。培养条件为光照强度1500 lx,光照时间12 h/d,温度25℃。南美天胡荽诱导不定芽分化的最佳培养基为MS+6-BA 0.12 mg/L+NAA 0.3 mg/L;生根的最佳培养基为MS+6-BA 0.3 mg/L+NAA 0.15 mg/L。

The aims were to study the effects of different culture conditions on regeneration status ofadventitious bud and rooting rate of Hydrocotyle vulgaris L., and screen the optimal culture condition. Usingauxiliary stems（about 1.0 cm） of Hydrocotyle vulgaris L. as explants, adventitious bud induction, multipleshoots proliferation and plant regeneration were the focal points in the research. The results showed that typeand mass concentration of growth regulator had obvious effects on sprouting time and regeneration rate ofadventitious bud. The proliferation rate was 89%, and the rooting rate was 98%. The culture condition wasillumination intensity 1500 lx, illumination time 12 h/d and culture temperature 25℃. The effective mediumfor cluster buds-inducing was MS＋6-BA 0.12 mg/L＋NAA 0.3 mg/L. The effective medium for rooting rate wasMS＋6-BA 0.3 mg/L＋NAA 0.15 mg/L.