燕麦AsNAC1耐盐基因的克隆及初步验证

Cloning and preliminary validation of the AsNAC1 gene for salt tolerance from oat

NAC转录因子家族对植物耐盐胁迫起着重要作用。该研究从燕麦中克隆了NAC转录因子家族基因As NAC1,并对其进行生物信息学分析。结果表明,该基因全长1 710 bp,编码320个氨基酸,等电点为6.62,分子量为35.4 k D。序列分析表明As NAC1与大麦和小麦KUP/HAK/KT家族亲缘关系较近,预测该蛋白无疏水跨膜结构域,位于细胞核概率较大。成功构建了p CAMBIA1301-As NAC1植物过表达载体,并转化拟南芥,通过比较盐胁迫条件下野生型拟南芥株系与转基因株系的表型得出,盐胁迫条件下,转基因种子萌发率为63%,而野生型为43%;3个转基因型植株是野生型植株根长的1.39倍、1.52倍和1.37倍,鲜重、干重的1.50倍和1.49倍,说明As NAC1的表达提高了拟南芥植株的耐盐性。为揭示NAC家族基因As NAC1在植物中的耐盐机制,同时为利用转基因技术提高植物耐盐能力奠定研究基础。

NAC transcription factor family plays an important role in salt tolerance of plants. In this study, the NAC transcription factor family gene As NAC1 was cloned from oats, and the bioinformatics analysis was carried out. The results showed that the gene was 1 710 bp, encoding 320 amino acids, the isoelectric point was 6.62, and the molecular weight was 35.4 k D. The sequence analysis showed that As NAC1 was closely related to the KUP/HAK/KT of barley and wheat, and predicted that the protein had no hydrophobic transmembrane domain. Successfully constructed the plant p CAMBIA1301- As NAC1 expression vector, and transformed into Arabidopsis thaliana, obtained by comparison of salt stress under the condition of wild type Arabidopsis lines and transgenic lines phenotype, salt stress conditions, transgenic seed germination rate was 63%, and wild type was 43%; transgenic plant was wild type plants root length 1.39 times, 1.52 times and 1.37 times, fresh weight and dry weight of 1.50 times and 1.49 times,it indicated that salt tolerance of Arabidopsis plants were improved As NAC1 the expression.In order to provide experimental basis for revealing the salt tolerance mechanism of As NAC1 family gene NAC in plants, the study laid a foundation for improving the ability of plant salt tolerance by transgenic technology.