腐殖质氧化还原官能团测定新方法

New methods for the determination of redox functional groups in humic substances

腐殖质是一种主要由碳、氢、氧、氮等元素构成的具有氧化还原活性的有机质,存在丰富的官能团,其中以酚基和羧基含量最为丰富.腐殖质的氧化还原能力与氧化还原官能团的数目和种类有关.一直以来醌基被认为是腐殖质最重要的氧化还原官能团,对于醌基的测定是研究氧化还原官能团的重点.对于腐殖质氧化还原官能团的研究以化学、电化学和微生物等基本方法为主.本文尝试将这些方法与光谱法(如三维荧光光谱法、红外光谱法、核磁共振光谱法和电子自旋共振波谱法)相结合,不仅可以量化腐殖质氧化还原能力,还能对腐殖质氧化还原官能团具体组成有完整的解释,以便全面了解腐殖质的氧化还原官能团和氧化还原活性.

Humic substances（ HS） are redox active organic matters which are mainly composed of carbon,hydrogen,oxygen,nitrogen and other elements. Reducing capacities of HS relate to the type and number of redox functional groups. HS contain abundant functional groups including phenol and carboxyl groups. Quinone moieties are generally considered as the most important redox functional groups. This study attempts to illustrate chemical,electrochemical and microbial methods to quantify the redox characteristics of humic substances,and combine spectroscopic techniques（ three-dimensional extraction-emission matrix technique,Fourier-transform infrared spectroscopy,nuclear magnetic resonance,electron spin resonance） to characterize the redox functional groups.The combination helps to better understand the composition of redox functional groups and the reducing capacities of HS.