不同强度三角波电磁场对大鼠骨密度和生物力学性能的影响

Effects of Electromagnetic Fields of Different Intensity on Bone Mineral Density and Biomechanical Properties of Rats

目的研究不同强度三角波电磁场(TEMF)对大鼠骨密度(BMD)和生物力学性能的影响。方法雌性SD大鼠54只随机分为6组,分别为对照组(0 m T)、0.1 m T、0.6 m T、1.2 m T、1.8 m T和2.4 m T组,每组9只。实验组每天使用TMEF干预90 min,对照组除不进行磁场干预外其他条件与实验组相同。在磁场干预第1、2、3月末腹腔麻醉测量BMD,并心脏取血处死,取离体的股骨和第4腰椎进行离体BMD检查、股骨三点弯曲和第4腰椎压缩实验。结果电磁场干预3个月0.1 m T组全身BMD值高于对照组(P<0.05);0.1 m T组离体股骨BMD、股骨弹性模量、最大载荷、屈服强度均高于对照组(P<0.05);6组第4腰椎弹性模量和最大载荷比较差异无统计学意义(P>0.05)。结论 0.1 m T TMEF能够提高大鼠全身BMD和股骨BMD,但在改善生物力学性能方面的效果不明显。

Objective To study effects of triangular wave electromagnetic field（ TEMF） of different intensity on bone mineral density（ BMD） and biomechanics ability on rats. Methods A total of 54 female SD rats were randomly divided into control group（ 0 mT）,0. 1 mT,0. 6 mT,1. 2 mT,1. 8 mT,and 2. 4 mT experimental groups（ n = 9 for each group） respectively. Experimental groups were treated with TEMF for 90 min daily,while control group received same treatments except for magnetic field intervention. BMD was measured by celiac anesthesia at the end of 1st,2ndand 3rd months of magnetic field treatment,and then rats were sacrificed by blood were taken from hearts. Isolated samples of femur and the fourth lumbar vertebrae were taken for BMD detection,three point bending and the fourth lumbar compression tests. Results After electromagnetic interference for 3 months,in 0. 1 MT group,value of whole body bone density was higher（ P〈0. 05）; values of isolated BMD of femur,femoral elastic modulus,maximum load and yield strength were higher than those in control group（ P〈0. 05）. There were no significant differences in the fourth lumbar elastic modulus and maximum load among the 6 groups and control group（ P〉0. 05）. Conclusion The 0. 1 mT TEMF can improve whole body and femur BMD,but it has poor effect on improving biomechanical properties.