补肾活血通络法对动脉粥样硬化易损斑块内VSMCS凋亡与RANTES水平相关性研究

Correlational Study of Bushen Huoxue Tongluo Method on the RANTES Expression Levels and VSMCs Apoptosis Ratio of the Stabilizing Atherosclerotic Vulnerable Plaque

目的:(1)观察补肾活血通络法对动脉粥样硬化易损斑块的稳定作用及其稳定斑块的可能机制;(2)探讨血清和斑块局部受激活调节正常T细胞表达和分泌因子(Regulated on Activated Normal T cell Expressed and Secreted factor,RANTES)水平与血管平滑肌细胞(vascular smooth muscle cells,VSMCs)凋亡程度的相关性。方法:将健康雄性新西兰兔34只,随机分成空白对照组(K组)和造模组(B组)。空白对照组8只,给予普通饲料喂养18周。造模组(B组)26只,给予高脂饲料喂养,实验开始第1周一次性注射牛血清白蛋白,实验第10周,随机抽取2只兔做病理检测证实模型组已形成粥样斑块。其余B组兔随机分为模型对照组、辛伐他汀组、健脑软脉方组。辛伐他汀组、健脑软脉方组分别予以辛伐他汀、健脑软脉方水煎剂干预,模型对照组予以生理盐水灌胃,持续8周,实验结束,于处死动物前24 h和48 h 2次给予药物触发易损斑块(中国斑点蝰蛇毒和组胺)。空白对照组(K组)按上述给药时间和部位给予同等剂量无菌生理盐水注射。实验终点处死实验兔,开胸迅速分离主动脉全长,检测各项指标。结果:模型对照组的主动脉病变程度、血脂水平、血清RANTES水平、斑块内RANTES m RAN和蛋白表达水平、VSMCs凋亡率与健脑软脉方组、辛伐他汀组、空白对照组相比有显著差异(P<0.05),健脑软脉方组和辛伐他汀组与空白对照组相比有显著差异(P<0.05),健脑软脉方组和辛伐他汀组之间无显著差异(P>0.05)。结论:本研究表明补肾活血通络法可能通过调节血脂水平,抑制炎症反应,抑制平滑肌细胞凋亡,从而抑制模型兔动脉粥样硬化斑块的不稳定性进展。

Objective：（1）To investigate the effect of Bushen Huoxue Tongluo method on stabilizing the atherosclerotic vulnerable plaque and to explore the possible mechanism of stabilizing the vulnerable plaqueby the animal experiment;（2）To study the correlations between the expression of RANTES（regulated on activated normal T cell expressed and secreted factor）and apoptosis of VSMCs（vascular smooth muscle cells）. Methods：34 of healthy male New Zealand white rabbits were randomized into the blank control group（group K,n=8）and the model group（group B,n=26）. Rabbits in the blank control group were fed with standard chow diet for 18 weeks. Rabbits in the model group were fed with the high cholesterol diet,injection of bovine serum albumin at first week. At 10 th week,two rabbits were randomly selected for pathological tests confirmed the model group has formed atherosclerotic plaque. The remaining group B rabbits were randomly divided into model control group,simvastatin group,Jiannao Ruanmai Decoction group. The simvastatin group were treated with simvastatin,Jiannao Ruanmai Decoction group were treated with Jiannao Ruanmai Decoction and the model control group with saline for 8 weeks. The end of the experiment,all of the remaining in the model group（group B）underwent pharmacological triggering with injection of Chinese Russell＇s viper venom and histamine at 24 h and 48 h before the rabbits were sacrificed,the blank control group（group K）were administered with the same dosage of sterile saline injection according the above time and location. At the end of the experiment the rabbits were sacrificed,separating a total length of aorta and detecting each index. Results：Lesions in the drug group compared with the model group was significantly reduced. The RANTES expression levels and VSMCs apoptosis ratio of Jiannao Ruanmai Decoction group and the simvastatin group was significantly reduced. Conclusion：Bushen Huoxue Tongluo method has effects of anti-inflammation,decreasing the level of lipid and leading to stabilize the vulnerable plaque（P〈0.05）.