蓬子菜水煎液对AS大鼠主动脉NF-κB表达及血清中ICAM-1、VCAM-1、P-seletin影响

Effects of Galium Verum Decoction on NF-κB Signaling Path Ways and the Expressions of ICAM-1,VCAM-1,P-seletin in the Serum on Atherosclerosis Model of Rats

目的:通过动物实验观察蓬子菜水煎液对动脉粥样硬化模型大鼠的影响,从抗炎及对NF-κB信号通路的影响等角度探讨蓬子菜水煎液对动脉硬化的作用机制。方法:通过复合方法建立了动脉粥样硬化模型大鼠。采用蓬子菜水煎液不同剂量灌胃治疗,通塞脉片灌胃作对照,应用免疫组化方法检测病变组织中NF-κB(P65)的表达;应用ELISA法检测ICAM-1、VCAM-1、P-seletin含量。结果:蓬子菜水煎液高中低剂量组NF-κB(P65)的水平与病理模型组及通塞脉片组相比有显著性差异(P<0.05)。蓬子菜水煎液组及通塞脉片组大鼠血清中ICAM-1、VCAM-1、P-seletin的表达与病理模型组相比显著下降,有显著性差异(P<0.05)。蓬子菜水煎液高剂量组与通塞脉片组有显著性差异(P<0.05)。结论:蓬子菜水煎液可降低NF-κB蛋白在病变组织中的表达,降低AS大鼠血清中ICAM-1、VCAM-1、P-seletin的水平,且优于通塞脉片组。

Objective：To explore the mechanism by which Galium verum Decoction worked on atherosclerosis from the perspective of anti-inflammation and effects on NF-κB signaling pathways by observing the effects of Galium verum Decoction on atherosclerosis model of rat. The conclusion preliminary proved the mechanism of inhibiting the AS formation. Methods：The AS rat model was established by composite methods. The model group was administered by intragastric with various doses of Galium verum Decoction,while the Tongsaimai Tablet was administered as the control group. Expressions of NF-κB in the diseased tissues were determined by immunohistochemical method,and the content of VCAM-1 was detected by ELISA method. Results：Compared with pathological group,level of NF-κB（P65）and expressions of ICAM-1,VCAM-1,P-seletin in the rats＇ serum was noticeable decreased in Galium verum Decoction group with low,middle and high dose,and had obviously differences comparing to Tongsaimai Tablet medicated group（P0.05）. Conclusions：Galium verum Decoction can decrease the NF-κB protein expression in the diseased tissues and decrease the level of ICAM-1,VCAM-1,P-seletin in serum of AS model rats.