盐酸异丙嗪对肝癌HepG2细胞增殖和凋亡的影响

Effect of Promethazine Hydrochloride on HepG2 Cell Proliferation and Apoptosis

目的:研究盐酸异丙嗪对肝癌HepG2细胞增殖和凋亡的影响。方法:采用不同浓度(15、23和31μmol/L)的盐酸异丙嗪处理HepG2细胞48 h,应用噻唑蓝(MTT)法测定细胞的增殖活性,检测乳酸脱氢酶(LDH)活性,Hoechst33258染色法检测细胞的凋亡情况。结果:盐酸异丙嗪能以浓度依赖的方式抑制HepG2细胞的增殖,23μmol/L盐酸异丙嗪处理48 h能够显著诱导HepG2细胞凋亡;对照组与处理组之间的LDH活性无显著性差异(P>0.05)。结论:盐酸异丙嗪可显著抑制肝癌HepG2细胞增殖,这与其诱导细胞凋亡途径有关。

Objective： To find out whether promethazine hydrochloride can inhibit hepatic cancer（HepG2 cell）and to explore the inhibition mechanism. Methods： The HepG2 cells have been treated by different concentrations（15, 23 and 31 μmol/L） of promethazine hydrochloride for 48 h. Then the proliferative activity was determinedby the MTT assay, and the apoptosis was determined by Hoechst 33258 staining assay. Lactate dehydrogenase（LDH） was also tested. Results： The proliferation of the HepG2 cells was significantly suppressed by the drug ina dose-dependent manner. However, there were no significant differences（P〉0.05） in the LDH release of the cellsbetween the control group and the drug treatment groups. Significant apoptosis was induced in HepG2 cells afterincubated with 23 μmol/L of promethazine hydrochloride for 48 h. Conclusion： Promethazine hydrochloride couldinhibit the proliferation of HepG2 cells by inducing cellular apoptosis. The drug may be a clue to find effectiveand safe drug for heptic cancer.