秀丽槭叶总黄酮的提取及其抗氧化能力研究

Extraction and Antioxidant Activities of Total Flavonoids From Acer elegantulum Fang. Leaves

为开发和利用秀丽槭叶中的黄酮资源,利用响应面分析法优化乙醇-超声法提取秀丽槭叶中总黄酮的工艺,并分析总黄酮清除DPPH自由基能力和总抗氧化能力。结果表明,在超声功率200 W条件下,秀丽槭叶总黄酮适宜的提取工艺条件为:提取温度55℃、乙醇浓度70%、液料比50∶1(v/w)、提取时间48 min,该条件下提取到的秀丽槭叶总黄酮含量为58.664 mg·g-1,秀丽槭叶总黄酮清除DPPH自由基的EC50为0.528 mg·L^(-1),清除率可达95.56%;秀丽槭叶总黄酮在相同条件下较L-抗坏血酸具有更强的总抗氧化力。本研究结果为今后秀丽槭叶黄酮资源的开发利用提供了一定的科学依据。

In order to develop and utilize resource of flavonoids in Acer elegantulum leaves,response surface analysis methodology（ RSM） was applied to optimize the ultrasonic-assisted extraction conditions of total flavonoids from Acer elegantulum leaves,and the antioxidant activities of total flavonoids were also investigated through the measurement of DPPH scavenging activity and total antioxidant capacity. The results under the experimental conditions showed that the optimal parameters for total flavonoids extraction process were determined as follows： extraction temperature 55℃,ethanol concentration 70%,liquid-solid 50∶ 1 and extraction time 48 min. The total flavonoids yield obtained under such conditions was 58. 664 mg·g-1. EC50 for total flavonoids on scavenging DPPH was 0. 528 mg·L-1 and the scavenging rate against DPPH free radicals could reached to 95. 56%. Total flavonoids in Acer elegantulum leaves maintained higher antioxidant capacity than L-ascorbic acid. In this regard,the optimum extraction conditions of total flavonoids in Acer elegantulum leaves were obtained,and the antioxidant activities of total flavonoids were measured as well,which would provide reliable scientific instruction on the exploitation of flavonoids resource in Acer elegantulum leaves.