红叶李花中总黄酮提取工艺及抑制α-葡萄糖苷酶活性研究

Extraction Process of Flavonoids From the Flower of Purple-leaf Plum and α-glucosidase Inhibition Activities

为进一步开发红叶李资源,筛选其活性成分,通过比较水煮沸、醇回流、超声、微波、闪式、超临界CO_2(SFE-CO_2)、复合酶酶解等提取方法对红叶李花中总黄酮含量的影响和对α-葡萄糖苷酶活性的抑制效果,确定最佳提取方法,并通过单因素试验和响应面法优选提取工艺。结果表明,复合酶酶解联合SFE-CO_2萃取工艺提取效率和生物活性最佳,SFE-CO_2萃取的总黄酮对α-葡萄糖苷酶抑制率达到96.09%,IC50为12.16μg·m L-1。最佳提取工艺为:酶解温度30℃,酶解时间3.5 h,酶用量0.10%,萃取压力30 MPa,乙醇浓度85%,萃取温度45℃,萃取时间60 min。此条件下的总黄酮得率为11.55%。本研究结果为红叶李花中总黄酮的开发与利用提供了技术参考。

In this paper,for further development of Prunus cerasifera Ehrh. cv. Atropurpurea Jacg. and drug screening of its active components, the optimal extracting method was selected from water boiling extraction, alcohol reflux extraction,supersonic extraction,microwave extraction,smashing tissue extraction,SFE-CO2 extraction and enzymatic hydrolysis based on the flower＇ s flavonoid content and their α-glucosidase inhibition activities. Based on the singlefactor experiments,the best extraction conditions were optimized by response surface methodology. The complex enzymeassisted SFE-CO2 extraction technology was the best method with the inhibitory rate of 96. 09% and IC50 of 12. 16μg·m L-1on the α-glucosidase. The optimal extraction conditions were determined as follows： enzymatic temperature was 30 ℃,enzymatic time was 3. 5 h,enzymatic dosage was 0. 10%,alcohol concentration was 85%,extraction temperature was 45 ℃ and extraction time was 60 min. Under the optimal conditions,the polysaccharides yield reached11. 55%. And this study could be helpful for the development and utillization of total flavonoid components from the flower of Prunus cerasifera Ehrh. cv. Atropurpurea Jacg