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血清饥饿诱发的前列腺癌DU145细胞反应

The cellular response of prostate cancer DU145 cells induced by serum starvation
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摘要 目的探讨血清饥饿诱发的前列腺癌DU145细胞适应性反应。方法血清培养DU145细胞24h后更换为无血清培养基。24、48、72h后收集细胞,分别应用MTT实验、细胞计数实验研究血清饥饿对细胞活力、增殖能力的影响;Real-time PCR和Western blot实验分别在mRNA水平和蛋白水平检测干预前后细胞上皮间质转化(Epithelial-Mesenchymal Transition,EMT)相关基因表达的变化。结果随着血清饥饿时间的延长,DU145细胞拉长呈细叶状,多中心聚合性生长;细胞活力、增殖能力受到明显抑制。Real-time PCR、Western blot结果均显示血清饥饿后E-钙黏蛋白(E-cadherin)表达升高,SNAIL、SLUG表达降低。结论为应对血清饥饿,DU145细胞发生了EMT的逆转,这可能是其适应性反应的一部分。 To explore the cellular adaptive response of human prostate cancer DU 145 cells induced by serum starvation. Methods Prostate cancer DU 145 cells were cultured in DMEMsupplemented in 10% FBS and then replaced with serum free medium. Cells were collected from serum and serum- free medium after 24,48 and 72 hours. The cell viability and multiplication capacity were detected using MTTand Cell Counting,respectively. RT- PCRand W estern Blotting are used to examine EMTrelated gene expression. Results W ith serum starvation morphological changes of DU 145 cells were observed. MTTand Cell counting results indicated that serum starvation inhibited cell proliferation significantly. Real- time PCRand W estern Blotting results showed that the expression of E- cadherin gene increased,while SN AIL,SLU G decreased. Conclusion In response to serum starvation,EMTreversion occurred in DU 145 cells and this performance may be a part of the adaptive response.
作者 李璐 张国安 侯森 崔文
机构地区 济南大学山东省医学科学院医学与生命科学学院 山东省医学科学院 济宁医学院司法鉴定中心 济宁医学院法医学与医学检验学院
出处 《济宁医学院学报》 2016年第5期309-313,共5页 Journal of Jining Medical University
基金 山东省高等学校科技计划项目(J13LK14) 山东省研究生教育创新计划项目(SDYY14014)
关键词 血清饥饿 DU145 EMT 细胞反应 Serum starvation DU145 EMT Cell reflection
分类号 R73-3 [医药卫生—肿瘤]
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参考文献17

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济宁医学院学报
2016年 第5期

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