神经干细胞静脉移植对大鼠脊髓损伤的修复作用

Effect of neural stem cells vein transplantation on repair of spinal cord injury rats

制备阴性对照病毒转染的传代神经干细胞(NSCs),同期制作大鼠脊髓损伤模型,随机分为实验组、假手术组、空白组,并对其进行术后行为学评分。3 d后取实验组再次手术,尾静脉输注已用阴性对照病毒标记的大鼠NSCs浓缩液。于1周和6周时对假手术组、实验组大鼠行10%福尔马林灌注,以固定的大鼠脊髓段落,福尔马林浸泡存放去除的脊髓段1 d,石蜡包埋后切片。获得大量尚未分化、悬浮生长的NSCs球。完成NSCs的传代。实验组的行为学评分结果比假手术组分值高。实验组大鼠脊髓损伤区脊髓空洞体积较假手术组小。初步证明注射NSCs液的大鼠恢复速度高于假手术组和空白组,NSCs移植能够减小脊髓损伤处的空洞体积和促进血管生长,从而促进脊髓损伤后神经功能的恢复。

Neural stem cell （NSCs） were taken from brain tissue of SPF newborn rat and NSCs transfected with virus were prepared. The model of rat spinal cord injury（SCI） was bulit by modified Allen method. All rats were divided into three groups, including SCI group, control group and blank group. Afterwards, every rat was going on behavioral score. After three days, rats of SCI group were injected with NSCs through caudal vein. After one week and three weeks, rats of SCI group and control group were perfused with 10% formalin until rats muscles spasmed and rats became stiff. Then, fixed spinal cords from rats were taken from rats and were fixed in＇formalin paraffin section. NSCs of rats were isolated and cellular pellets were observed suspending in the medium. These cells showed long-term proliferation in vitro. Behavioral score of SCI group was higher than that of the control group. What＇s more, cavity volume in the injury site of spinal cord of SCI group was smaller than that of the control group （0. 27 ±0. 09,0. 57 ±0. 10 respectively）. The transplantation of NSCs could accelerate recovery process of rats and promote recovery of neurological function after spinal cord injury through reducing cavity volume in the injury site of spinal cord and facilitating the growth of blood vessel.