壳聚糖-银纳米粒子抗菌棉纤维灭菌机理初探

Study on the sterilization mechanism of cotton fabrics coated with chitosan-based silver nanoparticle

以壳聚糖(CS)为保护剂和分散剂,液相还原法制备了水溶性银纳米粒子(Chitosan/Silver Nanoparticles,CS/Ag NPs),并通过化学键负载于商业用脱脂棉表面,制得表面负载CS/Ag NPs的抗菌棉纤维。研究表明硅烷偶联剂是甲苯体积的8%,第一次处理7h,第二次处理10h,可使棉纤维的银负载量达到最大。探讨了抗菌棉纤维对耐药性甲氧西林金黄色葡萄球菌、革兰氏阳性菌金黄色葡萄球菌、蜡状芽孢杆菌和革兰氏阴性菌大肠埃希氏菌的抗菌效果。实验结果表明,对上述细菌的抑制率都大于99.8%,具有很好的抗菌效果和持久抗菌性。短接触时间内,抗菌棉纤维能够完成吸附菌体和杀灭菌体2个过程。

The Ag nanoparticles （Ag NPs） were prepared with chitosan as protect and stable agent,and the Ag NPs were applied to cotton fabrics together by chemical bonding to obtain the durable antibacterial cotton fabrics. The research showed that the loading account of Ag NPs on cotton fabrics attained maximum when the volume of the crosslinking agent was 8% against the volume of toluene, the first and the second treating time were respectively 7h and 10h. Different bacterium,including methiciHin-resistam staphylococcus aureus （MRSA）, gram-positive bacteria Staphylococcus aureus （S. aureus） and Bacillus cereus （B. cereus） as well as gram-negative bacteria Escherichia coli （E. coli） were used to test the antibacterial activities and durable antimicrobial effect of finished cotton fabrics. The results indicated that the antibacterial rate of cotton fabrics was up to 99.80/oo against the aforementioned bacteria, the cotton fabrics showed great and durable antibacterial effect. Furthermore, it suggested that the antibacterial process of cotton fabrics may include adsorption and sterilization.