超声波处理对纤维素酶酶解竹粉效果影响的研究

Influence of ultrasonic treatment on cellulase hydrolysis of bamboo powder

为提高纤维素酶酶解竹粉效率,采用超声波处理竹粉。通过单因素试验分析确定超声波预处理竹粉的最优条件为:频率53kHz,功率180W,时间30min,浴比1∶10,温度30℃。用中性纤维素酶8000L酶解原竹粉和经超声波处理的竹粉,相同酶解条件下,超声波处理竹粉生成的还原糖量较原竹粉最大可提高18.05%。吸附性测试结果表明,超声波处理的竹粉对酶液的吸附性能略高于原竹粉。扫描电镜图片对比看出,经超声波处理的竹粉表层结构被破坏,整齐的纤维束结构变得杂乱松散,竹粉比表面积增加。而红外光谱测试结果表明,超声波处理并未引起竹粉官能团的明显变化。因此可初步认为超声波处理是一种温和而有效的预处理方式。

In order to improve the efficiency of cellulase hydrolysis bamboo powder, ultrasonic treatment was used to process the bamboo powders. The optimal ultrasonic pretreatment condition was determined through univariate analysis. The preferred process was carried out at 30℃ for 30min with the bath ratio of 1： 10,the frequency of 53kHz,and the power of 180W. Neutral cellulase 8000L was used to hydrolyze original bamboo powders and ultrasonic-pretreated bamboo pow ders. The bamboo powders pretreated by ultrasonic presented more reduced sugar yield than that from original bamboo powders under the same enzymatic hydrolysis conditions. The maximum increase reached 18.05 %. Protein adsorption test showed that more proteins could absorb on the bamboo powders treated by ultrasonic than original bamboo powders. SEM images showed that the surface structure of bamboo powders could be destroyed by ultrasonic treatment. The tight bundle structure becames loosen, so the effective surface area of bamboo powder was increased. The infrared spectra-test results re- vealed that ultrasonic treatment caused no significant changes in chemical components of bamboo powders. Therefore, it can be preliminarily concluded that the ultrasonic treatment was a gentle and effective method to improve enzyme hydrolysis of bamboo.