摘要
摘要：为探究嗜酸乳杆菌在小肠中黏附调控机制，嗜酸乳杆菌黏附与细胞壁分选酶及其相关蛋白的基因表达之间的关系。实验构建体外模型研究了不同pH培养条件下嗜酸乳杆菌的黏附能力、测定了分选酶基因和分选酶相关蛋白基因相对表达差异。实验结果表明：在pH5．5-7．5培养条件下，随着pH升高嗜酸乳杆菌菌体黏附性显著增加，srt基因及其相关蛋白的基因表达量显著上调。研究结果为pH对嗜酸乳杆菌的黏附效应的影响机制提供了相关理论依据。
To study the adhesion mechanism of Lactobacillus acidophflus in the gut and the relationship of between adhesion mechanism with sortase and sortase dependent proteins genes, fn vitro,the adherence was evaluated. Meanwhile,the srt gene and related genes expression were determinate under different pH.The results showed that the gene expression levels of srt and related proteins increased significantly with the range of pH from .5.5 to 7.5. Also,the adhesiveness of Lactobacillus acidophilus was also enhanced.It revealed some mechanisms of adhesion ability in Lactobacillus acidophilus upon different pH values.
作者
王刚
吴振
彭刘杨
潘道东
王文文
曾小群
WANG Gang WU Zhen PENG Liu-yang PAN Dao- dong WANG Wen-wen ZENG Xiao- qun(Key Laboratory of＂ Animal Protein Food Deep Processing Technology of Zhejiang Province, Ningbo University, Ningbo 315211, China Food Science ＆ Nutrition Department of Nanjing Normal University, Nanjing 210097, China)
出处
《食品工业科技》
CAS
CSCD
北大核心
2016年第21期166-171,共6页
Science and Technology of Food Industry
基金
国家自然科学基金面上项目(31471598
41276121)
浙江省自然科学基金青年基金项目(LQ16C200002)
江苏省自然科学基金项目(BK20141447)
国家科技部/星火计划(2015GA701043)
浙江省动物蛋白食品精深加工技术重点实验室开放基金(ZX2015000928)
学校人才工程项目(理)/人才引进(ZX2015000594)
关键词
嗜酸乳杆菌
PH
黏附性
分选酶
表达差异
Lactobacillus acidophilus
pH
adhesion
enzyme activity
differential expression
