响应面法优化酶解罗非鱼皮制备抗氧化肽的工艺研究

Optimization of preparation of Tilapia skin antioxidative peptides with enzymatic hydrolysis by response surface methodology

采用响应面分析法对酶法水解罗非鱼皮制备抗氧化肽的工艺条件进行优化。以水解度、DPPH自由基与OH自由基半抑制浓度(IC50)、还原力为评价指标,分析比较了商业蛋白酶中的酸性蛋白酶、中性蛋白酶、碱性蛋白酶、胰蛋白酶、菠萝蛋白酶及木瓜蛋白酶对罗非鱼皮的水解效果及其产物的抗氧化活性,筛选出碱性蛋白酶为最佳水解用酶,并利用响应面分析法对罗非鱼皮酶解条件予以了优化,最终确立的最佳酶解工艺为:p H10.00、反应时间4 h、温度40℃、料液比0.38 g/m L,在此条件下产物的还原力为1.054,与模型预测值相吻合,表明了响应面法的有效性。

Response surface methods were used to optimize the hydrolysis conditions of Tilapia skin for production of antioxidant peptides.Half inhibitory concentrations of DPPH radical, hydroxyl free radical and reducing power capacity were selected as the indexes to compare the effect of hydrolysis and product antioxidant activity of 6 kinds of commercial enzymes （ acid protease, neutral protease, alkaline protease, trypsin, bromelain and papain）. The result showed alkaline protease was optimum enzyme.Then the hydrolysis conditions was optimized by response surface methodology. The hydrolysis conditions were pill0.00, hydrolysis time of 4 h, hydrolysis temperature of 40 ℃, ratio of material to water of 0.38 g/mL.Under such conditions,the reducing power was 1.054, which was consistent with the model prediction.