野生枝瑚菌多糖的提取及单糖组成分析

Extraction and analysis of monosaccharide compositions of wild Ramaria sp.polysaccharide

为探索野生枝瑚菌多糖水浸提的最佳条件,在单因素实验基础上,采用合理的实验设计方案,应用响应面分析法优化野生枝瑚菌多糖的提取条件,并采用柱前衍生高效液相色谱法测定野生枝瑚菌多糖的单糖组成。根据回归分析得到野生枝瑚菌粗多糖的最佳提取条件为温度91℃,水料比32∶1(m L/g),时间3.2 h,在此条件下野生枝瑚菌多糖得率为1.97%。高效液相色谱分析结果表明枝瑚菌多糖由甘露糖、核糖、葡萄糖、半乳糖、木糖、岩藻糖六种单糖组成,各组分摩尔比为:45.8∶10.6∶76.4∶8.53∶4.63∶5.24。实验结果表明,响应面法对野生枝瑚菌多糖的提取条件优化合理可行,PMP-HPLC法,可用于测定野生枝瑚菌多糖中单糖的组成。

To optimize the extraction conditions for the polysaccharides from Ramaria sp., based on the single factor trials,the response surface methodology were applied.Using the high performance liquid chromatography for the determination of the monosaccharide composition.The results showed that the optimum extraction conditions was as follows ：temperature 91℃, water-to- material- ratio 32： 1 （ mL/g）, time 3.2 h. Under these conditions, the actual extaction rate was 1.97%.The PMP-HPLC results showed that the Ramaria sp.polysaccharides was composed of mannose, ribose,glucose, galatose,xylose, and fucose and their molar ratio was about 45.8： 10.6： 76.4： 8.53： 4.63： 5.24. Conclusion=The RSM method could be used for optimizing the extraction condition of Ramaria sp.polysaccharides and the PMP-HPLC method could be used for determination of polysaccharide compositions.