草菇子实体GC-MS分析及不同极性部位体外抗氧化活性研究

GC-MS analysis of Volvariella volvacea fruiting bodies and different solvent extracts antioxidant activities in vitro

目的:研究草菇乙醇提取物不同极性部分的抗氧化活性及子实体GC-MS分析。方法:用95%的乙醇回流提取草菇,依次用有机溶剂萃取获得石油醚层、氯仿层、乙酸乙酯层、正丁醇层4个不同极性部位。以2,6-二叔丁基对甲酚(BHT)为对照,分别考察其总抗氧化能力、DPPH自由基清除活性、铁离子还原能力、ABTS自由基清除活性,比较草菇乙醇提取物不同极性部位的抗氧化作用。结果:通过GC-MS分析,分析出49个化合物,以烷烃类、脂肪酸类、醛类、酯类、酮类为主,含量分别为22.54%、19.94%、15.38%、13.08%、7.33%。通过四种体外抗氧化能力的测定,草菇乙醇提取物的不同极性部位均有抗氧化活性,其中乙酸乙酯层和正丁醇层表现出较好的抗氧化活性,氯仿层的抗氧化活性最弱。在所设定浓度范围内,其乙酸乙酯层的总抗氧化能力、DPPH自由基清除率、铁离子还原能力明显高于其它层(p<0.05)。在ABTS自由基清除体系中,正丁醇层的ABTS自由基清除率高于乙酸乙酯层,但差异不显著(p>0.05)。结论:通过对草菇子实体GC-MS分析及抗氧化活性测试,草菇提取物的乙酸乙酯层和正丁醇层的抗氧化作用强,是天然抗氧化剂的良好来源,应对其进一步分离纯化。

Objective：To investigate antioxidant activities in vitro of different polarity fractions of ethanolextract and GC-MS analysis from Volvariella volvacea fruiting bodies.Methods： Volvariella volvacea was extracted with volume fraction of 95% ethanol reflux extraction, and decompressed concentrating to obtain ethanol extract, then four different polarity parts of Volvariella volvacea extracts were prepared by the addition of petroleum ether, chloroform,ethylacetate and butanol layer.The antioxidant activity in vitro of different polar parts of Volvariella volvacea extracts were investigated by the total antioxidant capacity, DPPH free radical scavenging activity, ferric reducing ability and ABTS radical scavenging activity, and 2,6-ditert butylated hydroxytoluene （BHT）was the controI.Results：49 compounds were analyzed from the Volvariella volvacea fruiting bodies by GC-MS analysis, which mainly included alkanes, fatty acids, aldehydes, esters, alcohols, ketones, and the content was respectively 22.54% ,19.94%, ]5.38%, 13.08%, 7.33% .Through the determination of four in vitro antioxidant capacity, straw mushroom ethanolic extracts of different polar parts all showed antioxidant activity,which layer of ethyl acetate and butanol layer showed good antioxidant activity and the chloroform layer of antioxidant activity was the lowest.Within the set concentration range, the total antioxidant capacity, DPPH free radical scavenging rate and iron ion reduction ability of the ethyl acetate layer were significantly higher than those of other layers（ p 〈 0.05）.1n the ABTS free radical scavenging system,the ABTS radical scavenging rate of the normal butanol layer was higher than that of the ethyl acetate layer, but the difference was not significant （ p 〉 0.05 ）. Conclusions. Through the analysis of GC-MS and test of antioxidant activity of the fruiting body,ethy lacetate fraction and normal butyl alcohol fraction, which were a kind of good source for natural antioxidants,showed relatively strong antioxidant activities,and were worth separating and purifying further.