EST-SSR标记在谷类作物中的通用性研究进展

Research progress in transferability of EST-SSR makers in grain crops

EST-SSR标记是基于表达序列标签(Express Sequence Tags,EST)开发简单重复序列(Simple Sequence Repeats,SSR)的一种与功能基因直接相关的新型分子标记技术,因其在不同作物间具有较好的通用性而被广泛应用于作物基因组学研究。不同谷类作物间EST-SSR的通用性可降低引物开发成本、提高效率,为遗传研究较薄弱的作物奠定基础。文章概括介绍了EST-SSR标记,对其在不同谷类作物间及相比较于G-SSR标记的通用性情况进行简单归类分析,评述了EST-SSR标记的不足及其优化措施,以期利用EST-SSR高通用性的特点推动谷类作物基因组学的发展。

EST-SSR is a new molecule marking technology to exploit Simple Sequence Repeats（SSR） based on Express Sequence Tags（ EST）.It was widely used in the study of crop genomics because of its good transferability in different crops. The transferability of EST-SSR in different grain crops could cut the costs of primers development and improve efficiency.It could lay the foundation of the crops that genetic studies are weak. EST- SSR makers and its transferability in different grain crops compared with G-SSR were introduced in this paper.The disadvantages and improvements in order to accelerate the development of crop genomics by using the high transferability of EST-SSR were reviewed.